A pilot study of the metabolomic profiles of saliva from female orthodontic patients with external apical root resorption

• Published in: Clinica chimica acta Vol. 478; pp. 188 - 193
• Main Authors: Zhou, Jinglin, Hu, Huimin, Huang, Renhuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2018
• Subjects: 1H NMR spectroscopy; Adult; Biomarkers - analysis; Early Diagnosis; Female; Humans; Inflammation; Metabolomics; Metabolomics - methods; Orthodontically induced external apical root resorption; Orthodontics - methods; Oxidative stress; Pilot Projects; Proton Magnetic Resonance Spectroscopy; Root Resorption - diagnosis; Saliva; Saliva - chemistry; Saliva - metabolism; Tooth Apex; Young Adult; Metabolomics; Oxidative stress; OIEARR; Orthodontically induced external apical root resorption; OPG; OTM; Inflammation; 1H NMR spectroscopy; TSP; NMR; ROS; EARR; Saliva; H NMR spectroscopy
• ISSN: 0009-8981; 1873-3492; 1873-3492
• DOI: 10.1016/j.cca.2017.12.046

Orthodontically induced external apical root resorption (OIEARR) is one of the most severe complications of orthodontic treatment, which is hard to diagnose at early stage by merely radiographic examination. This study aimed to identify salivary metabolic products using unbiased metabolic profiling in order to discover biomarkers that may indicate OIEARR. Unstimulated saliva samples were analyzed from 19 healthy orthodontic patients with EARR (n=8) and non-EARR (n=11). Metabolite profiling was performed using 1H Nuclear Magnetic Resonance (NMR) spectroscopy. A total of 187 metabolites were found in saliva samples. With supervised partial least squares discriminant analysis and regression analysis, samples from 2 groups were well separated, attributed by a series of metabolites of interest, including butyrate, propane-1,2-diol, α-linolenic acid (Ala), α-glucose, urea, fumarate, formate, guanosine, purine, etc. Indicating the increased inflammatory responses in the periodontal tissues possibly associated with energy metabolism and oxidative stress. The effective separation capacity of 1H NMR based metabolomics suggested potential feasibility of clinical application in monitoring periodontal and apical condition in orthodontic patients during treatment and make early diagnosis of OIEARR. Metabolites detected in this study need further validation to identify exact biomarkers of OIEARR. Saliva biomarkers may assist in diagnosis and monitoring of this disease. •Introduced metabolomic approach to investigate clinical orthodontic root resorption•Several salivary metabolite biomarkers may be related to orthodontic root resorption.•Saliva is easily accessible for clinical monitoring orthodontic root resorption.