Development of molecular detection kit for Larimichthys crocea and Larimichthys polyactis

• Published in: Biochip journal Vol. 8; no. 2; pp. 148 - 153
• Main Authors: Cho, Hyunseok, Kim, Ji-Hoon, Lee, Won Sun, Jeong, Woochang, Moon, Hyo-Bang, Hwang, Seung Yong
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean BioChip Society (KBCS) 01.06.2014; Springer Nature B.V; 한국바이오칩학회
• Subjects: Biomarkers; Biomedical Engineering and Bioengineering; Biotechnology; Chemistry; Chemistry and Materials Science; Dyes; Fisheries; Lab-on-a-chip; Larimichthys crocea; Larimichthys polyactis; Original Article; Polymerase chain reaction; Probes; Species; 생물공학; Real-time PCR; Mitochondrial 16S rRNA; Identification
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-014-8210-3

Large yellow croaker ( Larimichthys crocea ) and redlip yellow croaker ( L. polyactis ) are commonly consumed species in fisheries markets in East Asian countries. They have wide differences of price due to consumer preferences for L. polyactis . However, it is difficult to identify the species based on the appearance of both species from the general public. In this study, a real-time polymerase chain reaction (PCR) kit was designed to distinguish these two species. Speciescommon primer pairs were designed, and two speciesspecific probes were designed with sequences that were 2 bp different. The L. crocea target probe was modified using HEX dye, whereas the L. polyactis was modified using FAM dye. These primer pairs and probes worked in a single reaction. Based on the confirmation of a simple peak image, both species were sufficiently distinguished. The PCR kit developed in our study can be suggested as a good tool to distinguish both fish species for consumer as well as producer. In addition, the species-specific probes can be used as the biomarker, which applies to the lab-on-a-chip technology.