Quantitative lateral-flow immunoassay for the assessment of the cartilage oligomeric matrix protein as a marker of osteoarthritis

• Published in: Biochip journal Vol. 6; no. 3; pp. 213 - 220
• Main Authors: Hong, Sung Yub, Park, Yoo Min, Jang, Yo Han, Min, Byoung-Hyun, Yoon, Hyun C.
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean BioChip Society (KBCS) 01.09.2012; Springer Nature B.V; 한국바이오칩학회
• Subjects: Antibodies; Arthritis; Biomedical Engineering and Bioengineering; Biotechnology; Cartilage; Cartilage diseases; Cartilage oligomeric matrix protein; Chemistry; Chemistry and Materials Science; Enzyme-linked immunosorbent assay; Gold; Immunoassay; Matrix protein; Monoclonal antibodies; Nanoparticles; Original Research; Osteoarthritis; Proteins; Software; Strip; Synovial fluid; 생물공학; Synovial fluid; Osteoarthritis; Lateralflow immunoassay; Cartilage oligomeric matrix protein; Quantitative rapid kit
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-012-6303-4

This study reports a simple and quantitative analytical method for detecting cartilage oligomeric matrix protein (COMP) using a lateral-flow immunoassay (LFI) format. An immuno-chromatographic assay was developed using a gold nanoparticle (AuNP)-conjugated monoclonal antibody (Ab) probe for the quantitative detection of COMP in human synovial fluid (SF). The detection antibody was conjugated with the AuNP (∼30 nm) to enable detection, and the capture antibody was immobilized within a multi-spot pattern in the test zone of a membrane strip. COMP in the human SF binds to the AuNP-conjugated detection antibody, and the COMP-Ab complex then binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commercial scanner and an imaging software program. The color density of the multi-spot pattern at the test zone increased in proportion to the concentration of COMP. The developed immunoassay exhibited a dynamic detection range from 0.6 to 20 μg/mL of COMP. The detection limit of the proposed LFI system was approximately 0.2 μg/mL. The test results showed good correlation with those obtained using a conventional enzyme-linked immunosorbent assay (ELISA) to detect COMP. We expect that the proposed immunoassay method, based on the quantification of COMP, is suitable for osteoarthritis diagnosis using SF samples.