An electrodeposited Au nanoparticle/porous graphene nanoribbon composite for electrochemical detection of alpha-fetoprotein

• Published in: Materials chemistry and physics Vol. 242; p. 122514
• Main Authors: Jothi, Lavanya, Jaganathan, Saravana Kumar, Nageswaran, Gomathi
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.02.2020; Elsevier BV
• Subjects: Alpha fetoprotein; Antigens; Biomolecules; Chemical reduction; Chemical synthesis; Diagnosis; Differential pulse voltammetry; Electrochemical analysis; Electrodes; Electron transfer; Electron transport; Glassy carbon; Gold; Gold nanoparticles; Graphene; Immunosensors; Nanomaterials; Nanoparticles; Nanoribbons; Porous graphene nanoribbons; Alpha fetoprotein; Porous graphene nanoribbons; Gold nanoparticles; Differential pulse voltammetry
• ISSN: 0254-0584; 1879-3312
• DOI: 10.1016/j.matchemphys.2019.122514

In this work, a novel label-free electrochemical immunosensor for the detection of alpha-fetoprotein (AFP) is fabricated for early diagnosis and prognostics of liver cancer. A porous graphene nanoribbon (PGNR) was synthesized via chemical reduction method and it was electrodeposited with gold nanoparticles (AuNPs) to obtain AuNPs/PGNR hybrid nanomaterial. Anti-AFP was immobilized onto AuNPs/PGNR/glassy carbon electrode and anti-AFP/AuNPs/PGNR/GCE was further studied to demonstrate its electrocatalytic activity towards AFP antigen. PGNR enhances the electroactive surface area and the electron transfer ability between the electrode and redox probe while the AuNPs deposited on PGNR are used to immobilize biomolecules and to facilitate the electron transport. The superior biosensing performance towards AFP under physiological pH condition is demonstrated by a corresponding decreased peak current in differential pulse voltammetry for a wide linear range (5–60 ng/mL) with a low detection limit of 1 ng/mL. Detection of AFP in serum samples by this label-free electrochemical immunosensor without fouling or significant interference implies that the anti-AFP/AuNPs/PGNR modified GCE has a great application potential for clinical diagnosis of AFP. [Display omitted] •A novel porous graphene nanoribbon was obtained via chemical reduction method.•AuNPs electrodeposited on porous graphene nanoribbon acts as platform for immobilizing biomolecule.•Porous graphene nanoribbon/AuNPs can be used for detection of AFP in human serum.