Assessment of Artemisia annua extracts contribution to viral stress tolerance in Ficus carica L.: physiological and molecular contribution

• Published in: Discover life Vol. 55; no. 1; p. 15
• Main Authors: Aboelhasan, Fatma M. O., Sobhy, Sherien E., Soliman, Hemaid I. A., Saleh, Ahmed A., Hafez, Elsayed E.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.12.2025; Springer Nature B.V
• Subjects: Agricultural practices; Agricultural research; Antiviral activity; Antiviral agents; Artemisia annua; Astronomy; Astrophysics and Astroparticles; Biochemistry; biomarkers; Biomedical and Life Sciences; Biotechnology; Carbonyl compounds; Cultivars; Cultivation; Culture techniques; Desert regions; dose response; Earth Sciences; Egypt; Ficus carica; Fig mosaic emaravirus; Fruit cultivation; Fruit trees; fruits; Gene expression; Global economy; Hydrogen peroxide; Life Sciences; Middle East; Observations and Techniques; Oxidative stress; Plant extracts; plant health; Plant propagation; Plants; Propagation; stress tolerance; Sustainable production; Tissue culture; Tubulin; Viruses; Middle East; Egypt; Oxidative stress; Biotic stress; L; Artemisia extract; Viral infection; Molecular response
• ISSN: 2948-2976; 0169-6149; 2948-2976; 1573-0875
• DOI: 10.1007/s11084-024-09673-2

Ficus carica L. (Fig) is one of the earliest cultivated fruit trees and is extensively grown in the Mediterranean, Middle East, and Egypt’s desert regions. Despite its importance, Fig cultivation faces challenges, including low propagation success rates and Fig Mosaic Virus ( FMV ) infections. This study aimed to overcome these hurdles through biotechnological approaches and the use of Artemisia annua extract. Virus-free Fig plants were generated from infected ‘Sultani’ cultivars using tissue culture techniques. Additionally, the antiviral properties of A. annua extract against FMV were assessed. The extract markedly reduced oxidative stress biomarkers, such as MDA , carbonyl, and H 2 O 2 , and decreased the expression levels of defence-related genes, including PAL , PPO , PPX , PR3 , PR5 and Tubulin , in a dose-dependent manner, with concentrations of 200 and 400 µl/l showing the most significant effects. Results indicated a reduction of 40–60% in oxidative stress markers and a general reduction in gene expression fold changes in virus-infected samples treated with the extract compared to controls. These findings highlight the potential of integrating biotechnological techniques and plant-derived compounds to enhance Fig cultivation, ensuring sustainable production and improved plant health. The successful propagation of virus-free Fig plants and the efficacy of A. annua extract in combating FMV showcase promising methodologies to address current propagation difficulties and viral threats in Fig agriculture. This research underscores the importance of biotechnological innovations and the use of natural antiviral agents in advancing agricultural practices for global economic benefits.