Arthrobacter liuii sp. nov., resuscitated from Xinjiang desert soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. Pt_3; pp. 896 - 901
• Main Authors: Yu, Xiao-Yun, Zhang, Li, Ren, Biao, Yang, Na, Liu, Mei, Liu, Xue-Ting, Zhang, Li-Xin, Ding, Lin-Xian
• Format: Journal Article
• Language: English
• Published: England International Union of Microbiological Societies 01.03.2015
• Subjects: Actinobacteria; Arthrobacter; Arthrobacter - classification; Arthrobacter - genetics; Arthrobacter - isolation & purification; Bacterial Typing Techniques; Base Composition; catalase; Cell Wall - chemistry; cell walls; China; Desert Climate; desert soils; DNA; DNA, Bacterial - genetics; fatty acids; Fatty Acids - chemistry; Lysine - chemistry; Molecular Sequence Data; Nucleic Acid Hybridization; nucleotide sequences; Phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; soil sampling; China
• ISSN: 1466-5034; 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.000037

A Gram-stain positive, aerobic, non-motile actinobacterium, designated DSXY973 ᵀ, was isolated from soil samples collected from Xinjiang desert using medium supplemented with resuscitation-promoting factor, and subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 16S rRNA gene sequences revealed that DSXY973 ᵀ belonged to the genus Arthrobacter and was most closely related to Arthrobacter oryzae JCM 15922 ᵀ with 97.1 % similarity. The DNA G+C content was 67.6 %. Cells of strain DSXY973 ᵀ mainly contained MK-9(H ₂), and the cell wall contained l -lysine as the primary diamino acid. The major cellular fatty acids were anteiso-C ₁₅ : ₀, anteiso-C ₁₇ : ₀ and iso-C ₁₅ : ₀. Strain DSXY973 ᵀ was positive for catalase and negative for oxidase activity. On the basis of its phylogenetic position and phenotypic properties, strain DSXY973 ᵀ represents a novel species of the genus Arthrobacter , for which the name Arthrobacter liuii sp. nov. is proposed. The type strain is DSXY973 ᵀ (= CGMCC1.12778 ᵀ = JCM 19864 ᵀ).