Evaluation of the Potential Risk of Porcine Endogenous Retrovirus (PERV) Infection in Nude Mice

• Published in: Journal of microbiology and biotechnology Vol. 21; no. 4; pp. 387 - 390
• Main Authors: Bae, E.H., Dankook University, Cheonan, Republic of Korea, Jung, Y.T., Dankook University, Cheonan, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul Korean Society for Applied Microbiology 01.04.2011; 한국미생물·생명공학회
• Subjects: Animal model; Animals; Biological and medical sciences; Biotechnology; Cell Line; CELLS; CELLULE; CELULAS; Disease Models, Animal; Endogenous Retroviruses - genetics; Endogenous Retroviruses - isolation & purification; Endogenous Retroviruses - physiology; Fundamental and applied biological sciences. Psychology; Gammaretrovirus - genetics; Gammaretrovirus - physiology; Humans; Mice; Mice, Inbred BALB C; Mice, Nude; nude mice; porcine endogenous retroviruses; replication-defective retroviral vector; Retroviridae Infections - transmission; Retroviridae Infections - veterinary; Retroviridae Infections - virology; Swine; Swine Diseases - transmission; Swine Diseases - virology; Transplantation, Heterologous; xenotransplantation; 생물학; Animal model; Rodentia; Retroviridae; xenotransplantation; Heterotransplantation; Pig; Virus; Infection; replication-defective retroviral vector; Vertebrata; Mammalia; Mouse; porcine endogenous retroviruses; Replication; nude mice; Artiodactyla; Vector; Ungulata
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1012.12013

Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.