Characterization of the Lsi1 Homologs in Cucurbita moschata and C. ficifolia for Breeding of Stock Cultivars Used for Bloomless Cucumber Production

• Published in: Weon'ye gwahag gi'sulji Vol. 35; no. 3; pp. 333 - 343
• Main Authors: Jung, Jaemin, Kim, Joonyup, Jin, Bingkui, Choi, Youngmi, Hong, Chang Oh, Lee, Hyun Ho, Kang, Jumsoon, Park, Younghoon
• Format: Journal Article
• Language: English
• Published: 한국원예학회HST 01.01.2017; 한국원예학회
• Subjects: 농학; cucurbitaceae; Lsi2; transporters; marker-assisted breeding; Low silicon rice 1; RACE PCR
• ISSN: 1226-8763; 2465-8588
• DOI: 10.12972/kjhst.20170036

Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks(Cucurbita moschata ) to restricted silicon (SiO2) absorption. Inhibition of silicon absorptionin bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata ) and its coldtolerantwild relative C. ficifolia (‘Heukjong’) in order to develop a DNA marker for selectinga bloomless trait and to establish the molecular basis for breeding bloomless stock cultivarsof C. ficifolia . A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) wasdesigned based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of theCmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) wasconfirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. QuantitativeRT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1gene does not appear to be associated with the bloom/bloomless trait and may differ dependingon plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1 (B+)]of ‘Heukjong’ (C. ficifolia ), was cloned and sequence comparison between CmLsi1 (B+) andCfLsi1 (B+) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1 (B+) and Lsi1 homologs further revealed that CfLsi1 (B+) isclosesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata . KCI Citation Count: 4