Production of human interferon ALPHA 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression
Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10³ IU/g fresh wei...
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| Published in | Cytology and genetics Vol. 44; no. 5; pp. 313 - 316 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Heidelberg
Heidelberg : Allerton Press, Inc
01.10.2010
Allerton Press, Inc |
| Subjects | |
| Online Access | Get full text |
| ISSN | 0095-4527 1934-9440 |
| DOI | 10.3103/S0095452710050099 |
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| Abstract | Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10³ IU/g fresh weight (FW) with an average of 2.1 ± 0.8 × 10³ IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b. |
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| AbstractList | Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10³ IU/g fresh weight (FW) with an average of 2.1 ± 0.8 × 10³ IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b. Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10 3 IU/g fresh weight (FW) with an average of 2.1 ± 0.8 × 10 3 IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium -mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b. |
| Author | Gerasymenko, I. M Kuchuk, N. V Spivak, N. Y Sheludko, Y. V Sindarovska, Y. R Olevinskaya, Z. M |
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| Cites_doi | 10.1007/s004380050604 10.1016/j.scienta.2006.12.047 10.1016/j.vaccine.2005.01.006 10.2174/187220808786241033 10.1016/S0168-1656(01)00229-2 10.1016/S0958-1669(00)00236-6 10.1016/j.biochi.2007.05.001 10.1016/S1360-1385(01)01922-7 10.1002/bit.21075 10.1016/j.vaccine.2004.11.011 10.1073/pnas.0400149101 10.1016/S0958-1669(02)00303-8 10.1111/j.1467-7652.2007.00258.x 10.1007/s11248-004-2376-8 10.1073/pnas.96.2.703 10.1016/0003-2697(76)90527-3 10.1111/j.1467-7652.2008.00344.x 10.1073/pnas.96.20.11128 10.1089/10799900152547858 10.1001/jama.199.8.519 10.1111/j.1467-7652.2006.00216.x 10.1016/S0167-7799(02)00007-0 10.1046/j.1365-313X.2003.01676.x 10.1128/JVI.37.2.755-758.1981 10.1002/bit.22652 10.1001/jama.1967.03120080053007 10.1111/j.1467-7652.2005.00154.x 10.1089/jir.1992.12.449 10.5511/plantbiotechnology.23.91 |
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| References | Chelbi-Alix, Wietzerbin (CR1) 2007; 89 Sheludko, Sindarovska, Gerasymenko, Bannikova, Kuchuk (CR24) 2007; 96 McCormick, Kumagai, Hanley (CR20) 1999; 96 Bradford (CR27) 1976; 72 Ohya, Matsumura (CR5) 2001; 21 Voinnet, Rivas, Mestre, Baulcombe (CR25) 2003; 33 Arazi, Slutsky, Shiboleth (CR8) 2001; 87 Chen, Lin (CR4) 2004; 13 Sawahel (CR6) 2002; 6 Ma, Drake, Chargelegue, Obregon, Prada (CR12) 2005; 23 Maliga (CR17) 2003; 21 Masumura, Morita (CR3) 2006; 23 Arlen, Falconer (CR7) 2007; 5 Moore, Gerner, Franklin (CR30) 1967; 199 Rubinstein, Familletti, Pestka (CR28) 1981; 37 Stoger, Sack, Fischer, Christou (CR11) 2002; 13 Larrick, Thomas (CR14) 2001; 12 Edelbaum, Stein (CR2) 1992; 12 Huang, Phoolcharoen, Lai, Piensook, Cardineau, Zeitlin, Whaley, Arntzen, Mason, Chen (CR13) 2009; 106 Schob, Kunz, Meins (CR26) 1997; 256 Streatfield (CR16) 2007; 5 Belotskii, Spivak (CR29) 2006 Vaquero, Sack, Chandler, Drossard, Schuster, Monecke, Schillberg, Fischer (CR19) 1999; 96 CR21 Daniell, Streatfield, Wycoff (CR15) 2001; 6 Song, Zhao (CR10) 2008; 9 Li, Chen (CR9) 2007; 112 Marillonnet, Giritch, Gils, Kandzia, Klimyuk, Gleba (CR22) 2004; 101 Sheludko (CR18) 2008; 2 Benchabane, Goulet, Rivard, Faye, Gomord, Michaud (CR31) 2008; 6 Gleba, Klimyuk, Marillonnet (CR23) 2005; 23 6117_CR21 L. Song (6117_CR10) 2008; 9 G.E. Moore (6117_CR30) 1967; 199 S. Marillonnet (6117_CR22) 2004; 101 H. Schob (6117_CR26) 1997; 256 Y. Gleba (6117_CR23) 2005; 23 P.A. Arlen (6117_CR7) 2007; 5 Y.V. Sheludko (6117_CR24) 2007; 96 E. Stoger (6117_CR11) 2002; 13 C. Vaquero (6117_CR19) 1999; 96 A.A. McCormick (6117_CR20) 1999; 96 O. Edelbaum (6117_CR2) 1992; 12 S.J. Streatfield (6117_CR16) 2007; 5 O. Voinnet (6117_CR25) 2003; 33 M.M. Bradford (6117_CR27) 1976; 72 P. Maliga (6117_CR17) 2003; 21 T.L. Chen (6117_CR4) 2004; 13 Z. Huang (6117_CR13) 2009; 106 T. Masumura (6117_CR3) 2006; 23 S. Rubinstein (6117_CR28) 1981; 37 J. Li (6117_CR9) 2007; 112 Y.V. Sheludko (6117_CR18) 2008; 2 J.K. Ma (6117_CR12) 2005; 23 W.A. Sawahel (6117_CR6) 2002; 6 M. Benchabane (6117_CR31) 2008; 6 K. Ohya (6117_CR5) 2001; 21 J.W. Larrick (6117_CR14) 2001; 12 S.M. Belotskii (6117_CR29) 2006 T. Arazi (6117_CR8) 2001; 87 H. Daniell (6117_CR15) 2001; 6 M.K. Chelbi-Alix (6117_CR1) 2007; 89 |
| References_xml | – volume: 256 start-page: 581 year: 1997 end-page: 585 ident: CR26 article-title: Silencing of Transgenes Introduced into Leaves by Agroinfiltration: a Simple, Rapid Method for Investigating Sequence Requirements for Gene Silencing publication-title: Mol. Gen. Genet. doi: 10.1007/s004380050604 – volume: 112 start-page: 258 year: 2007 end-page: 265 ident: CR9 article-title: Transient Expression of an Active Human Interferon-beta in Lettuce publication-title: Sci. Hort. doi: 10.1016/j.scienta.2006.12.047 – volume: 23 start-page: 91 year: 2006 end-page: 97 ident: CR3 article-title: Production of Biologically Active Human Interferon-α in Transgenic Rice publication-title: Plant Biotechnol. – volume: 23 start-page: 2042 year: 2005 end-page: 2048 ident: CR23 article-title: Magnifection—A New Platform for Expressing Recombinant Vaccines in Plants publication-title: Vaccine doi: 10.1016/j.vaccine.2005.01.006 – volume: 2 start-page: 198 year: 2008 end-page: 208 ident: CR18 article-title: Agrobacterium-Mediated Transient Expression as an Approach to Production of Recombinant Proteins in Plants publication-title: Recent Patents Biotechnol. doi: 10.2174/187220808786241033 – volume: 37 start-page: 755 issue: 5 year: 1981 end-page: 758 ident: CR28 article-title: Convenient Assay for Interferons publication-title: J. Virol. – volume: 87 start-page: 67 year: 2001 end-page: 82 ident: CR8 article-title: Engineering Zucchini Yellow Mosaic Potyvirus as a Non-Pathogenic Vector for Expression of Heterologous Proteins in Cucurbits publication-title: J. Biotechnol. doi: 10.1016/S0168-1656(01)00229-2 – volume: 12 start-page: 411 year: 2001 end-page: 418 ident: CR14 article-title: Producing Proteins in Transgenic Plants and Animals publication-title: Curr. Opin. Biotechnol. doi: 10.1016/S0958-1669(00)00236-6 – volume: 89 start-page: 713 year: 2007 end-page: 718 ident: CR1 article-title: Interferon, a Growing Cytokine Family: 50 Years of Interferon Research publication-title: Biochimie doi: 10.1016/j.biochi.2007.05.001 – volume: 6 start-page: 219 year: 2001 end-page: 226 ident: CR15 article-title: Medical Molecular Farming: Production of Antibodies, Biopharmaceuticals and Edible Vaccines in Plants publication-title: Trends Plant Sci. doi: 10.1016/S1360-1385(01)01922-7 – volume: 96 start-page: 608 year: 2007 end-page: 614 ident: CR24 article-title: Comparison of Several Nicotiana Species as Hosts for High-Scale -Mediated Transient Expression publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.21075 – volume: 23 start-page: 1814 year: 2005 end-page: 1818 ident: CR12 article-title: Antibody Processing and Engineering in Plants, and New Strategies for Vaccine Production publication-title: Vaccine doi: 10.1016/j.vaccine.2004.11.011 – volume: 101 start-page: 6852 year: 2004 end-page: 6857 ident: CR22 article-title: In Planta Engineering of Viral RNA Replicons: Efficient Assembly by Recombination of DNA Modules Delivered by Agrobacterium publication-title: Proc. Nat. doi: 10.1073/pnas.0400149101 – volume: 13 start-page: 161 year: 2002 end-page: 166 ident: CR11 article-title: Plantibodies: Applications, Advantages and Bottlenecks publication-title: Curr Opin Biotechnol. doi: 10.1016/S0958-1669(02)00303-8 – volume: 5 start-page: 511 year: 2007 end-page: 525 ident: CR7 article-title: Field Production and Functional Evaluation of Chloroplast-Derived Interferon-alpha 2b publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2007.00258.x – volume: 13 start-page: 499 year: 2004 end-page: 510 ident: CR4 article-title: Expression of Bioactive Human Interferon-gamma in Transgenic Rice Cell Suspension Cultures publication-title: Transgenic Res. doi: 10.1007/s11248-004-2376-8 – volume: 96 start-page: 703 year: 1999 end-page: 708 ident: CR20 article-title: Rapid Production of Specific Vaccines for Lymphoma by Expression of the Tumor-Derived Single-Chain Fv Epitopes in Tobacco Plants publication-title: Proc. Nat. Acad. Sci. USA doi: 10.1073/pnas.96.2.703 – ident: CR21 – volume: 72 start-page: 248 year: 1976 end-page: 254 ident: CR27 article-title: A Rapid and Sensitive Method for the Quantification of Microgram Quantities of Protein Utilizing the Principle to Protein-Dye Binding publication-title: Anal. Biochem. doi: 10.1016/0003-2697(76)90527-3 – volume: 6 start-page: 633 year: 2008 end-page: 648 ident: CR31 article-title: Preventing Unintended Proteolysis in Plant Protein Biofactories publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2008.00344.x – volume: 96 start-page: 11128 issue: 20 year: 1999 end-page: 11133 ident: CR19 article-title: Transient Expression of a Tumor-Specific Single-Chain Fragment and a Chimeric Antibody in Tobacco Leaves publication-title: Proc. Nat. Acad. Sci. USA doi: 10.1073/pnas.96.20.11128 – volume: 21 start-page: 595 year: 2001 end-page: 602 ident: CR5 article-title: Expression of Two Subtypes of Human IFN-alpha in Transgenic Potato Plants publication-title: J. Interferon. Cytokine Res. doi: 10.1089/10799900152547858 – volume: 199 start-page: 519 year: 1967 end-page: 524 ident: CR30 article-title: Culture of Normal Human Leukocytes publication-title: J. Amer. Med. Ass. doi: 10.1001/jama.199.8.519 – volume: 106 start-page: 9 year: 2009 end-page: 17 ident: CR13 article-title: High-Level Rapid Production of Full-Size Monoclonal Antibodies in Plants by a Single-Vector DNA Replicon System publication-title: Biotechnol. Bioeng. – volume: 5 start-page: 2 year: 2007 end-page: 15 ident: CR16 article-title: Approaches to Achieve High-Level Heterologous Protein Production in Plants publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2006.00216.x – volume: 12 start-page: 449 year: 1992 end-page: 453 ident: CR2 article-title: Expression of Active Human Interferon-beta in Transgenic Plants publication-title: J. Interferon Res. – volume: 21 start-page: 20 year: 2003 end-page: 28 ident: CR17 article-title: Progress Towards Commercialization of Plastid Transformation Technology publication-title: Trends Biotechnol. doi: 10.1016/S0167-7799(02)00007-0 – volume: 33 start-page: 949 year: 2003 end-page: 956 ident: CR25 article-title: An Enhanced Transient Expression System in Plants Based on Suppression of Gene Silencing by the P19 Protein of Tomato Bushy Stunt Virus publication-title: Plant J. doi: 10.1046/j.1365-313X.2003.01676.x – volume: 6 start-page: 19 year: 2002 end-page: 29 ident: CR6 article-title: The Production of Transgenic Potato Plants Expressing Human alpha-Interferon Using Lipofectin-Mediated Transformation publication-title: Cell Mol. Biol. Lett. – year: 2006 ident: CR29 publication-title: Interferony: biologicheskie i klinicheskie effekty – volume: 9 start-page: 351 year: 2008 end-page: 355 ident: CR10 article-title: Transient Expression of Chicken alpha Interferon Gene in Lettuce publication-title: J. Zhejiang Univ. Sci. B – volume: 6 start-page: 219 year: 2001 ident: 6117_CR15 publication-title: Trends Plant Sci. doi: 10.1016/S1360-1385(01)01922-7 – volume: 37 start-page: 755 issue: 5 year: 1981 ident: 6117_CR28 publication-title: J. Virol. doi: 10.1128/JVI.37.2.755-758.1981 – volume: 21 start-page: 595 year: 2001 ident: 6117_CR5 publication-title: J. Interferon. Cytokine Res. doi: 10.1089/10799900152547858 – volume: 96 start-page: 703 year: 1999 ident: 6117_CR20 publication-title: Proc. Nat. Acad. Sci. USA doi: 10.1073/pnas.96.2.703 – volume: 106 start-page: 9 year: 2009 ident: 6117_CR13 publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.22652 – volume: 96 start-page: 608 year: 2007 ident: 6117_CR24 publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.21075 – volume: 96 start-page: 11128 issue: 20 year: 1999 ident: 6117_CR19 publication-title: Proc. Nat. Acad. Sci. USA doi: 10.1073/pnas.96.20.11128 – volume: 87 start-page: 67 year: 2001 ident: 6117_CR8 publication-title: J. Biotechnol. doi: 10.1016/S0168-1656(01)00229-2 – volume: 2 start-page: 198 year: 2008 ident: 6117_CR18 publication-title: Recent Patents Biotechnol. doi: 10.2174/187220808786241033 – volume: 6 start-page: 19 year: 2002 ident: 6117_CR6 publication-title: Cell Mol. Biol. Lett. – volume-title: Interferony: biologicheskie i klinicheskie effekty year: 2006 ident: 6117_CR29 – volume: 199 start-page: 519 year: 1967 ident: 6117_CR30 publication-title: J. Amer. Med. Ass. doi: 10.1001/jama.1967.03120080053007 – volume: 112 start-page: 258 year: 2007 ident: 6117_CR9 publication-title: Sci. Hort. doi: 10.1016/j.scienta.2006.12.047 – volume: 256 start-page: 581 year: 1997 ident: 6117_CR26 publication-title: Mol. Gen. Genet. doi: 10.1007/s004380050604 – volume: 9 start-page: 351 year: 2008 ident: 6117_CR10 publication-title: J. Zhejiang Univ. Sci. B – volume: 101 start-page: 6852 year: 2004 ident: 6117_CR22 publication-title: Proc. Nat. doi: 10.1073/pnas.0400149101 – ident: 6117_CR21 doi: 10.1111/j.1467-7652.2005.00154.x – volume: 23 start-page: 1814 year: 2005 ident: 6117_CR12 publication-title: Vaccine doi: 10.1016/j.vaccine.2004.11.011 – volume: 13 start-page: 161 year: 2002 ident: 6117_CR11 publication-title: Curr Opin Biotechnol. doi: 10.1016/S0958-1669(02)00303-8 – volume: 21 start-page: 20 year: 2003 ident: 6117_CR17 publication-title: Trends Biotechnol. doi: 10.1016/S0167-7799(02)00007-0 – volume: 12 start-page: 449 year: 1992 ident: 6117_CR2 publication-title: J. Interferon Res. doi: 10.1089/jir.1992.12.449 – volume: 13 start-page: 499 year: 2004 ident: 6117_CR4 publication-title: Transgenic Res. doi: 10.1007/s11248-004-2376-8 – volume: 5 start-page: 2 year: 2007 ident: 6117_CR16 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2006.00216.x – volume: 33 start-page: 949 year: 2003 ident: 6117_CR25 publication-title: Plant J. doi: 10.1046/j.1365-313X.2003.01676.x – volume: 72 start-page: 248 year: 1976 ident: 6117_CR27 publication-title: Anal. Biochem. doi: 10.1016/0003-2697(76)90527-3 – volume: 23 start-page: 91 year: 2006 ident: 6117_CR3 publication-title: Plant Biotechnol. doi: 10.5511/plantbiotechnology.23.91 – volume: 5 start-page: 511 year: 2007 ident: 6117_CR7 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2007.00258.x – volume: 12 start-page: 411 year: 2001 ident: 6117_CR14 publication-title: Curr. Opin. Biotechnol. doi: 10.1016/S0958-1669(00)00236-6 – volume: 89 start-page: 713 year: 2007 ident: 6117_CR1 publication-title: Biochimie doi: 10.1016/j.biochi.2007.05.001 – volume: 23 start-page: 2042 year: 2005 ident: 6117_CR23 publication-title: Vaccine doi: 10.1016/j.vaccine.2005.01.006 – volume: 6 start-page: 633 year: 2008 ident: 6117_CR31 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2008.00344.x |
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| Snippet | Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved... Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved... |
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| SubjectTerms | Biomedical and Life Sciences Biomedicine genes Human Genetics humans interferons Nicotiana proteins signal peptide |
| Title | Production of human interferon ALPHA 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression |
| URI | https://link.springer.com/article/10.3103/S0095452710050099 https://www.proquest.com/docview/1539447266 |
| Volume | 44 |
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