Production of human interferon ALPHA 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression

Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10³ IU/g fresh wei...

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Published inCytology and genetics Vol. 44; no. 5; pp. 313 - 316
Main Authors Sindarovska, Y. R, Gerasymenko, I. M, Sheludko, Y. V, Olevinskaya, Z. M, Spivak, N. Y, Kuchuk, N. V
Format Journal Article
LanguageEnglish
Published Heidelberg Heidelberg : Allerton Press, Inc 01.10.2010
Allerton Press, Inc
Subjects
Biomedical and Life Sciences
Biomedicine
genes
Human Genetics
humans
interferons
Nicotiana
proteins
signal peptide
Human Interferon
Plastid Transformation
Transgenic Potato Plant
Interferon
Transient Expression
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ISSN0095-4527
1934-9440
DOI10.3103/S0095452710050099

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Summary:Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 × 10³ IU/g fresh weight (FW) with an average of 2.1 ± 0.8 × 10³ IU/g FW. It proves that N. excelsior is a suitable host for Agrobacterium-mediated transient expression of genes encoding physiologically active human proteins. The transient expression conditions optimized for GFP marker protein were confirmed to be preferable for hIFN α2b.
Bibliography:http://dx.doi.org/10.3103/S0095452710050099
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ISSN:0095-4527
1934-9440
DOI:10.3103/S0095452710050099