Association of the miR-149 Rs2292832 Polymorphism with Papillary Thyroid Cancer Risk and Clinicopathologic Characteristics in a Chinese Population

• Published in: International journal of molecular sciences Vol. 15; no. 11; pp. 20968 - 20981
• Main Authors: Wei, Wen-Jun, Lu, Zhong-Wu, Li, Duan-Shu, Wang, Yu, Zhu, Yong-Xue, Wang, Zhuo-Ying, Wu, Yi, Wang, Yu-Long, Ji, Qing-Hai
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.11.2014; MDPI
• Subjects: Adult; Aged; Asian Continental Ancestry Group - genetics; Carcinoma - epidemiology; Carcinoma - genetics; Carcinoma - pathology; Carcinoma, Papillary; China - epidemiology; Female; Gene Expression Regulation, Neoplastic; Genetic Predisposition to Disease; Genotype; Genotype & phenotype; Humans; Male; MicroRNAs; MicroRNAs - genetics; Middle Aged; Polymorphism; Polymorphism, Genetic; Polymorphism, Single Nucleotide; Population genetics; Risk factors; Thyroid cancer; Thyroid Cancer, Papillary; Thyroid Gland - metabolism; Thyroid Gland - pathology; Thyroid Neoplasms - epidemiology; Thyroid Neoplasms - genetics; Thyroid Neoplasms - pathology; China
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms151120968

(1) Background: The genetic predisposition to papillary thyroid cancer (PTC) is far from clearly elucidated. Rs2292832 is a genetic polymorphism that located in the precursor of mir-149 and has been studied in diverse cancers. Thus far, the role of rs2292832 in PTC tumorigenesis and progression was unclear; (2) Method: Rs2292832 was genotyped in 838 PTCs, 495 patients with thyroid benign tumors (BNs) and 1006 controls in a Chinese Han population. Clinicopathological data was collected and compared. The expression level of mature mir-149 was examined in 55 normal thyroid tissue samples; (3) Results: The CC genotype of rs2292832 was significantly associated with an increased risk of PTC compared with TT homozygote (OR = 1.60, 95% CI: 1.72–2.20, p = 0.003) and TT/TC combined genotype (OR = 1.54, 95% CI: 1.14–2.09, p = 0.005). Rs2292832 is an independent risk factor correlated with tumor invasion (p = 0.006) and higher T stage in PTC patients (p = 0.007), but uncorrelated with short-term disease persistence of PTC. PTC subjects carrying CC genotype have lower mir-149-5p expression than those with TC genotype (p = 0.002). Twelve predicted target genes have been identified by collaboratively using computational tools; (4) Conclusion: Rs2292832 was possibly involved in the susceptibility and local progression of PTC in Chinese patients, by altering the expression level of mir-149-5p and its target genes.