Synthesis of size-controllable Fe3O4 magnetic submicroparticles and its biocompatible evaluation in vitro

• Published in: Journal of Central South University Vol. 23; no. 11; pp. 2784 - 2791
• Main Authors: Tian, Qing-hua, Ning, Wen-bo, Wang, Wei-jia, Yuan, Xiu-hong, Bai, Zhi-ming
• Format: Journal Article
• Language: English
• Published: Changsha Central South University 01.11.2016; Springer Nature B.V
• Subjects: Biocompatibility; Chemical and Environmental Engineering; Controllability; Engineering; Ferric chloride; Hemolysis; In vitro methods and tests; Iron oxides; Magnetic properties; Materials; Metallic Materials; Metallurgy; Sodium acetate; Synthesis; magnetite; submicroparticles; biocompatibility; hemolysis; cytotoxicity
• ISSN: 2095-2899; 2227-5223
• DOI: 10.1007/s11771-016-3341-4

Large scaled uniform and size-controllable magnetic submicroparticles (MSPs) were synthesized via solvothermal method with ferric chloride as iron source and sodium acetate as trapping agent. The influence of Fe 3+ and NaAc contents on the size distribution of MSPs was investigated. The structural and morphological properties of the synthesized particles were studied by scanning electron microscopy (SEM), X-ray power diffraction (XRD) and vibrating sample magnetometer (VSM). The well-dispersed MSPs with size of 100-1000 nm were obtained by simply adjusting the contents of Fe 3+ and NaAc. In addition, the hemolysis and cytotoxicity of Fe 3 O 4 MSPs, and their ability to case arrest in cell life-cycles were studied. The results indicate that larger size could lead to lower hemolysis. From MTT(3-(4,5-dimethylthuazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the interactions between MSPs and adhesive mouse fibroblast cell line(L929) were probed. Larger size of Fe 3 O 4 MSPs demonstrates lower cell viability following an exposure to the cells.