Powder Formulation Using Heat Resistant Endospores of Two Multi-functional Plant Growth Promoting Rhizobacteria Bacillus Strains Having Phytophtora Blight Suppression and Growth Promoting Functions
Multi-functional plant growth promoting rhizobacteria Bacillus subtilis AH18 and Bacillus licheniformis K11 have been shown to display synergic effects in promoting the growth of red peppers and in suppressing phytophtora blight and have a competitive relationship in a limited nutritional environmen...
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Published in | Applied biological chemistry Vol. 53; no. 4; pp. 485 - 492 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer-Verlag
01.08.2010
Springer Nature B.V 한국응용생명화학회 |
Subjects | |
Online Access | Get full text |
ISSN | 1738-2203 2468-0834 2234-344X 2468-0842 |
DOI | 10.3839/jksabc.2010.074 |
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Summary: | Multi-functional plant growth promoting rhizobacteria
Bacillus subtilis
AH18 and
Bacillus licheniformis
K11 have been shown to display synergic effects in promoting the growth of red peppers and in suppressing phytophtora blight and have a competitive relationship in a limited nutritional environment. In the present study, a powder formulation method using a commercially cheap and easily obtainable carrier was developed to formulate these excellent antagonistic
Bacillus
species into a product. When the endospores of both strains were simultaneously formulated using a synthetic zeolite of NaY and natural zeolite, the growth rate of the vegetative form of
B. sublitis
AH18 was 5×10
9
CFU/mL. In contrast, the growth of
B. licheniformis
K11 was suppressed when this formulation was used. However, when the synthetic zeolite of NaA was used in the simultaneous formulation, the survival rates of both bacteria at 45°C for 60 days was sustained and the bacterial concentration was the same as the initial injection concentration of 5×10
9
CFU/mL. Furthermore, when the growth rates of the liquid state endospore preparations at different pH values were examined for 60 days, the concentration of both the
Bacillus
species were shown to slowly decrease at pH 5.0 and pH 9.0, but under strong basic conditions (pH 11.0), both the bacteria maintained stable endospore counts. When the effects of the prototype formulated using the NaA carrier at pH 11.0 was investigated in
in vivo
pot tests, the formulation was shown to completely suppress the phytophtora blight and have an excellent growth promoting effect. Both the
Bacillus
species colonized the rhizosphere of red peppers for 15 days with more than 10
8
CFU/g. Therefore, the carrier formulation method developed in the present study using
Bacillus
endospores will allow for long storage of the product and can eliminate the competitive relationship between the two
Bacillus
species. In addition, if the formulation is commercially applied, several antagonistic microorganisms can be formulated into a single product, which is expected to be convenient for the manufacturing processes as well as increase the stability of the biological control agents for use in farmhouses. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 G704-000111.2010.53.4.018 |
ISSN: | 1738-2203 2468-0834 2234-344X 2468-0842 |
DOI: | 10.3839/jksabc.2010.074 |