Neuroprotective Effects of Phlorotannin-Rich Extract from Brown Seaweed Ecklonia cava on Neuronal PC-12 and SH-SY5Y Cells with Oxidative Stress

• Published in: Journal of microbiology and biotechnology Vol. 30; no. 3; pp. 359 - 367
• Main Authors: Nho, Jin Ah, Shin, Yong Sub, Jeong, Ha-Ram, Cho, Suengmok, Heo, Ho Jin, Kim, Gun Hee, Kim, Dae-Ok
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Microbiology and Biotechnology 28.03.2020; 한국미생물·생명공학회
• Subjects: Animals; Antioxidants - pharmacology; Cell Line; Humans; Neurons - drug effects; Neuroprotective Agents - pharmacology; Oxidative Stress - drug effects; PC12 Cells; Rats; Research article; Seaweed - chemistry; 생물학; neuron; dieckol; neuroprotection; Cholinesterase; vitamin C equivalent antioxidant capacity
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1910.10068

Neurodegenerative disorders in the elderly are characterized by gradual loss of memory and cognitive function. Oxidative stress caused by reactive oxygen species is associated with progressive neuronal cell damage and death in Alzheimer's disease, one of the most common neurodegenerative disorders. An edible brown seaweed, , contains a variety of biologically active compounds such as phlorotannins. In this study, we comparatively evaluated the total phenolic content, antioxidant capacity, and neuroprotective effects of the phlorotannin-rich extract from (PEEC). The total phenolic content of PEEC and dieckol was 810.8 mg gallic acid equivalents (GAE)/g and 996.6 mg GAE/g, respectively. Antioxidant capacity of PEEC was 1,233.8 mg vitamin C equivalents (VCE)/g and 392.1 mg VCE/g determined using ABTS and DPPH assays, respectively, while those of dieckol were 2,238.4 mg VCE/g and 817.7 mg VCE/g. High-performance liquid chromatography results revealed 48.08 ± 0.67 mg dieckol/g of PEEC. PEEC had neuroprotective effects in pheochromocytoma (PC-12) and human neuroblastoma (SH-SY5Y) cells against H O - and AAPH-induced oxidative damage, partly due to reduced intracellular oxidative stress. PEEC treatment inhibited acetylcholinesterase and butyrylcholinesterase in a dose-dependent manner. Taken together, these findings suggest that PEEC is a good source of antioxidants and neuroprotective materials.