In vitro metabolic studies on propyl p-nitrophenyl ether

• Published in: Biochimica et biophysica acta Vol. 237; no. 1; pp. 21 - 27
• Main Authors: Mitoma, C., Dehn, R.L., Tanabe, M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.04.1971
• Subjects: 1-Propanol - chemical synthesis; 1-Propanol - metabolism; Animals; Carbon Isotopes; Deuterium - pharmacology; Enzyme Induction; Ethers - chemical synthesis; Ethers - metabolism; Guinea Pigs; In Vitro Techniques; Male; Metabolism - drug effects; Methylcholanthrene - pharmacology; Microsomes, Liver - drug effects; Microsomes, Liver - enzymology; Nitrophenols - chemical synthesis; Nitrophenols - metabolism; Phenobarbital - pharmacology; Propionates - chemical synthesis; Propionates - metabolism; Rats; PNPE
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/0304-4165(71)90026-2

1. 1. This study was conducted to examine how the metabolic rates at the three sites on propyl p-nitrophenyl ether (PNPE) varied with respect to each other under various experimental conditions. The hepatic microsomal system of the rat and guinea pig showed differences in some respects. 2. 2. The rates of metabolism at the three sites varied independently of each other after pretreatment of the rat or the guinea pig with phenobarbital or 3-methyl-cholanthrene. Moreover, the major metabolite in these two species was different after enzyme induction, although (ω-2)-alcohol was the major metabolite in both species before induction. 3. 3. Substitution of hydrogen atoms with deuterium atoms at each of the metabolic sites resulted in selective inhibition of metabolism at the particular site. A general trend for a slightly increased formation of other metabolites was observed to compensate for the decreased formation of the major metabolite in both animal systems. Implications of these findings with regard to the number of enzymes involved in the metabolism of PNPE is discussed.