Lanthanum Chloride Promoted Proliferation with Enhanced S-phase Entry and Inhibited Potassium Currents of NIH 3T3 Cells

• Published in: Chinese journal of chemistry Vol. 29; no. 7; pp. 1411 - 1416
• Main Author: 张丽平 李卓玉 杨频
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2011; WILEY‐VCH Verlag; Wiley Subscription Services, Inc
• Subjects: 3T3细胞; Cell cycle; Cell growth; Ions; Lanthanum; NIH; NIH 3T3 cells; patch clamp technique; potassium current; Rodents; Synergistic effect; 增强型; 氯化镧; 浓度依赖性; 细胞增殖; 胚胎成纤维细胞; 钾电流
• ISSN: 1001-604X; 1614-7065
• DOI: 10.1002/cjoc.201180247

The effects of La^3＋ on proliferation, cell cycles, apoptosis and ion channels were investigated in mouse embryo fibroblast NIH 3T3 cells and its possible mechanisms were explored. Our data showed that La^3＋ promoted cell proliferation with increased S-phase entry and inhibited the outward potassium currents in a concentration-dependent manner in NIH 3T3 cells. La^3＋ and Ca^2＋ had synergistic effect on cell proliferation and cell cycles. It showed that Ca^2＋ was needed for La^3＋ promoted cell cycle progression. Using the whole-cell voltage-clamp technique, we found that La^3＋ blocked the outward potassium current in a Lanthanum ions can increase intracellular Ca^2＋ concentration concentration-dependent manner in NIH 3T3 cells. through inhibition of potassium currents, which induce a series of physiological changes and improve proliferation of cells. This may be one of the molecular mechanisms of lanthanum ions induced cell proliferation. The present work provides a new perspective for understanding the biological and toxicological effects of lanthanum.