Efficacy of Veronica incana for Treating Osteoarthritis Induced by Monosodium Iodoacetate in Rats

• Published in: Journal of medicinal food Vol. 26; no. 6; p. 379
• Main Authors: Lee, Jin A, Ngo, Trung Huy, Shin, Mi-Rae, Choi, Jeong Won, Choi, Hyukjae, Nam, Joo-Won, Roh, Seong-Soo
• Format: Journal Article
• Language: English
• Published: United States 01.06.2023
• Subjects: anti-inflammatory; Veronica incana; MIA; osteoarthritis
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2023.K.0001

The aim of this study is to investigate the efficacy and the underlying mechanism of in osteoarthritis (OA) induced by intraarticular injection of monosodium iodoacetate (MIA). The selected major four compounds (A-D) of were found from fractions 3 and 4. Its structure elucidation was determined by liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) data analysis and nuclear magnetic resonance (NMR) data comparison with literature. MIA (50  L with 80 mg/mL) for the animal experiment was injected into the right knee joint. The was administered orally every day to rats for 14 days from 7 days after MIA treatment. Finally, we confirmed the four compounds: (A) verproside; (B) catalposide; (C) 6-vanilloylcatapol; and (D) 6-isovanilloylcatapol. When we evaluated the effect of on the MIA injection-induced knee OA model, there were a noticeable initial decreased in hind paw weight-bearing distribution compared to the Normal group (  < .001), but supplementation resulted in a significant increase in the weight-bearing distribution to the treated knee (  < .001). Moreover, the treatment led to a decrease in the levels of liver function enzymes and tissue malondialdehyde (  < .05 and .01). The significantly suppressed the inflammatory factors through the nuclear factor-kappa B signaling pathway and downregulated the expression of matrix metalloproteinases, which are involved in the degradation of the extracellular matrix (  < .01 and .001). In addition, we confirmed the alleviation of cartilage degeneration through tissue stains. In conclusion, this study confirmed the major four compounds of and suggested that could serve as an anti-inflammatory candidate agent for patients with OA.