COMPARATIVE STUDY OF RANDOM AMPLIFIED POLYMORPHIC DNA OF TRYPANOSOMA CRUZI ISOLATES FROM PARAGUAY
The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established st...
Saved in:
| Published in | Japanese Journal of Tropical Medicine and Hygiene Vol. 27; no. 3; pp. 427 - 431 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japanese Society of Tropical Medicine
1999
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 0304-2146 2186-1811 2186-1811 |
| DOI | 10.2149/tmh1973.27.427 |
Cover
| Abstract | The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established strains isolated from Brazil, Chile and Colombia in South America. Of 9 Paraguayan isolates, 3 isolates were similar to the Berenice, Y and Sao Felipe strains (zymodeme type; Brazilian Z2), respectively, isolated from Brazil, and 4 strains were similar to the Tulahuen strain from Chile. There was no isolate similar to the Colombiana I strain (Z1) from Colombia. |
|---|---|
| AbstractList | The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established strains isolated from Brazil, Chile and Colombia in South America. Of 9 Paraguayan isolates, 3 isolates were similar to the Berenice, Y and Sao Felipe strains (zymodeme type; Brazilian Z2), respectively, isolated from Brazil, and 4 strains were similar to the Tulahuen strain from Chile. There was no isolate similar to the Colombiana I strain (Z1) from Colombia. |
| Author | BABA, SAYUMI SAKAMOTO, MAKOTO MALDONADO, MARISEL MIMORI, TATSUYUKI MATSUMOTO, TAMAMI KANBARA, HIROJI ARIAS, ANTONIETA ROJAS DE SAYA, HIDEYUKI |
| Author_xml | – sequence: 1 fullname: MIMORI, TATSUYUKI organization: Department of Tumor Genetics and Biology, Kumamoto University School of Medicine – sequence: 1 fullname: MALDONADO, MARISEL organization: Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncion – sequence: 1 fullname: BABA, SAYUMI organization: Department of Tumor Genetics and Biology, Kumamoto University School of Medicine – sequence: 1 fullname: SAKAMOTO, MAKOTO organization: Sanwa Hospital – sequence: 1 fullname: SAYA, HIDEYUKI organization: Department of Tumor Genetics and Biology, Kumamoto University School of Medicine – sequence: 1 fullname: MATSUMOTO, TAMAMI organization: Ginkyo College of Medical Science – sequence: 1 fullname: ARIAS, ANTONIETA ROJAS DE organization: Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncion – sequence: 1 fullname: KANBARA, HIROJI organization: Department of Protozoology, Institute of Tropical Medicine, Nagasaki University |
| BookMark | eNqFkEtvgkAUhSdNm9Rat13PHwDngQMsJzyUBBzCowndkIEOVYNowKbx3xejMd11c8_ift9ZnBfw2B06BcAbRjrBhj0_7TfYNqlOTN0g5gOYEGwxDVsYP4IJosjQRow9g9kw7BBClGDbwmgCpCOimCc8C949mGa5W0Dhw4SvXRFBHsVh4AeeC2MRFpFI4lXgQHfNL0yWFDFfi1REHDpJ_hHAIBUhz7wU-skoX1qXOS9ewVMj20HNbjkFue9lzkoLxTJweKjVlGJTW6AFYgZDkjH0aTeWNI2FVTFDMYJVbaAxK4qIoqyyGSaUKdRQu0GGTRtJZEWnYH7t_e6O8vwj27Y89tu97M8lRuVlpPI2UknMchxpNPSrUfeHYehV87_gXIXdcJJf6o7L_rStW_UXv53Run_rjexL1dFf6FR51Q |
| ContentType | Journal Article |
| Copyright | Japanese Society of Tropical Medicine |
| Copyright_xml | – notice: Japanese Society of Tropical Medicine |
| DBID | AAYXX CITATION ADTOC UNPAY |
| DOI | 10.2149/tmh1973.27.427 |
| DatabaseName | CrossRef Unpaywall for CDI: Periodical Content Unpaywall |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| Database_xml | – sequence: 1 dbid: UNPAY name: Unpaywall url: https://proxy.k.utb.cz/login?url=https://unpaywall.org/ sourceTypes: Open Access Repository |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 2186-1811 |
| EndPage | 431 |
| ExternalDocumentID | 10.2149/tmh1973.27.427 10_2149_tmh1973_27_427 article_tmh1973_27_3_27_3_427_article_char_en |
| GroupedDBID | 5GY AAFWJ ALMA_UNASSIGNED_HOLDINGS JSF JSH KQ8 RJT RZJ AAYXX CITATION ADTOC UNPAY |
| ID | FETCH-LOGICAL-c3317-50506460a660d9f8a7458b64e621ec40e62b302e36b961236e0f39f0493fa2ab3 |
| IEDL.DBID | UNPAY |
| ISSN | 0304-2146 2186-1811 |
| IngestDate | Wed Oct 01 15:46:10 EDT 2025 Tue Jul 01 01:32:48 EDT 2025 Wed Sep 03 06:29:50 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | false |
| IsScholarly | false |
| Issue | 3 |
| Language | English |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c3317-50506460a660d9f8a7458b64e621ec40e62b302e36b961236e0f39f0493fa2ab3 |
| OpenAccessLink | https://proxy.k.utb.cz/login?url=https://www.jstage.jst.go.jp/article/tmh1973/27/3/27_3_427/_pdf |
| PageCount | 5 |
| ParticipantIDs | unpaywall_primary_10_2149_tmh1973_27_427 crossref_primary_10_2149_tmh1973_27_427 jstage_primary_article_tmh1973_27_3_27_3_427_article_char_en |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 1900 |
| PublicationDate | 1999 |
| PublicationDateYYYYMMDD | 1999-01-01 |
| PublicationDate_xml | – year: 1999 text: 1999 |
| PublicationDecade | 1990 |
| PublicationTitle | Japanese Journal of Tropical Medicine and Hygiene |
| PublicationTitleAlternate | Jap. J. Trop. Med. Hyg. |
| PublicationYear | 1999 |
| Publisher | Japanese Society of Tropical Medicine |
| Publisher_xml | – name: Japanese Society of Tropical Medicine |
| References | 1) Avila, H., Goncalves, A.M., Nehme, N.S., Morel, C.M. and Simpson, L. (1990) : Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences. Mol. Biochem. Parasitol., 42,175-188 5) Carreno, H., Rojas, C., Aguilera, X., Apt, W., Miles, M. and Solari, A. (1987) : Schizodeme analysis of Trypanosoma cruzi zymodemes from Chile. Exp. Parasitol., 64,252-260 10) Lewicka, K., Breniere, S., Barnabe, C., Wet, J.P. andTibayrenc, M. (1995) : An isoenzyme survey of Trypanosoma cruzi genetic variability in sylvatic cycles from french guiana. Exp. Parasitol., 81, 20-28 8) Gomes, M.L., Macedo, A.M., Pena, S.D. and Chiari, E. (1998) : Genetic relationships between Trypanosoma cruzi strains isolated from chronic chagasic patients in southern Brazil as revealed by RAPD and SSR-PCR analysis. Acta Trop., 69, 99-109 3) Canese, A. (1978) : Datos actualizados soble conocimientos epidemiologicos de la enfermedad de Chagas en el Paraguay. Revista Paraguaya de Microbiol., 13, 3-6 17) Tibayrenc, M., Echalar, L., Dujardin, J.P., Poch, 0. and Desjeux, P. (1984) : The microdistribucion of isoenzymic strains of Trypanosoma cruzi in southern Bolivia; new isoenzyme profiles and further arguments against Mendelian sexuality. Trans. R. Soc. trop. Med. Hyg., 78,519-525 2) Brisse, S., Barnabe, C., Banuls, A.L., Sidibe, I., Noel, S. and Tibayrenc, T. (1998) : A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting. Mol. Biochem. Parasitol., 92,253-256 4) Canese, A., Adorno, A., Cattoni, A., De Vargas, H., Galeano, A. and Sanchez, A. (1972) : Alteraciones electrocardiogrficas en pacientes con immunofluorescencia positiva para in enfermedad le Chages. Rev. Paraguaya de Microbiol., 7, 15-16 12) Miles, M.A. and Cibulskis, R.E. (1986) : The heterogeneity of Trypanosoma cruzi zymodeme characterization of Trypanosoma cruzi. Parasitol. Today, 2, 94-97 18) Tibayrenc, M. and Ayala, F.J. (1988) : Isozyme variability in T. cruzi, the agent of Chagas disease : Genetical, toxonomical and epidemiological significance. Evolution, 42,277-292 7) Ebert, F. (1982) : The identification of two maingroups of Trypanosoma cruzi stocks from Brazil by their isoenzyme patterns of isoelectrofocusing. Tropenmed. Parasitol., 33,140-146 11) Miles, M.A., Povoa, M., Prata, A., Cedillos, R., DeSouza, A.A. and Macedo, V. (1981) : Do radically dissimilar Trypanosoma cruzi strains (zymodemes) cause Venezuelan and Brazilian forms of Chagas' disease? Lancet, 8234, 1338-1340 14) Mimori, T., Sasaki, J., Nakata, M., Gomez, E.A., Uezato, H., Nonaka, S., Hashiguchi, Y., Furuya, M. and Saya, H. (1998) : Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene, 210,179-186 15) Rojas de Arias, A., Monzon, M.I., Velazques, G., Guillen, E. and Torreani, T.N. (1984) : A seroepidemiologic survey of Chagas' disease in two Paraguayan villages. Bull. Pan Am. Heal. Org., 18,164-171 19) Vidigal, T.H.D.A., Neto, E.D., Carvalho, O.D.S.C. and Simpson, A.J.G. (1994) : Biomphalaria glabrata : Extensive genetic variation in Brazillian isolates revealed by random amplified polymorphic DNA analysis. Exp. Parasitol., 79,187-194 6) Dice, L.R. (1945) : Measures of the amount of ecological association between species. Ecology, 26,853-860 9) Higo, H., Yanagi, T., Matta, V., Agatsuma, T., Kanbara, H., Tada, I., De Leon, M.P., Monroy, C. and Tabaru, Y. (1997) : Genetic Structure of Trypanosoma cruzi in Central America and its comparison with South America strains. Inter. J. Parasitol., 27, 1369-1374 13) Mimori, T., Maldonado, M., Samudio, M., Rojas de Arias, A., Moreno, R. and Sakamoto, M. (1992) : Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA. Annal. Trop. Med. Parasitol., 86,231-237 16) Steindel, M., Neto, E.D., De Menezes, C.L.P., Romanha, A.J. and Simpson, A.J.G. (1993) : Random amplified polymorphic DNA analysis of Trypanosoma cruzi strains. Mol. Biochem. Parasitol., 60, 71-80 |
| References_xml | – reference: 15) Rojas de Arias, A., Monzon, M.I., Velazques, G., Guillen, E. and Torreani, T.N. (1984) : A seroepidemiologic survey of Chagas' disease in two Paraguayan villages. Bull. Pan Am. Heal. Org., 18,164-171 – reference: 18) Tibayrenc, M. and Ayala, F.J. (1988) : Isozyme variability in T. cruzi, the agent of Chagas disease : Genetical, toxonomical and epidemiological significance. Evolution, 42,277-292 – reference: 6) Dice, L.R. (1945) : Measures of the amount of ecological association between species. Ecology, 26,853-860 – reference: 2) Brisse, S., Barnabe, C., Banuls, A.L., Sidibe, I., Noel, S. and Tibayrenc, T. (1998) : A phylogenetic analysis of the Trypanosoma cruzi genome project CL Brener reference strain by multilocus enzyme electrophoresis and multiprimer random amplified polymorphic DNA fingerprinting. Mol. Biochem. Parasitol., 92,253-256 – reference: 17) Tibayrenc, M., Echalar, L., Dujardin, J.P., Poch, 0. and Desjeux, P. (1984) : The microdistribucion of isoenzymic strains of Trypanosoma cruzi in southern Bolivia; new isoenzyme profiles and further arguments against Mendelian sexuality. Trans. R. Soc. trop. Med. Hyg., 78,519-525 – reference: 11) Miles, M.A., Povoa, M., Prata, A., Cedillos, R., DeSouza, A.A. and Macedo, V. (1981) : Do radically dissimilar Trypanosoma cruzi strains (zymodemes) cause Venezuelan and Brazilian forms of Chagas' disease? Lancet, 8234, 1338-1340 – reference: 1) Avila, H., Goncalves, A.M., Nehme, N.S., Morel, C.M. and Simpson, L. (1990) : Schizodeme analysis of Trypanosoma cruzi stocks from South and Central America by analysis of PCR-amplified minicircle variable region sequences. Mol. Biochem. Parasitol., 42,175-188 – reference: 10) Lewicka, K., Breniere, S., Barnabe, C., Wet, J.P. andTibayrenc, M. (1995) : An isoenzyme survey of Trypanosoma cruzi genetic variability in sylvatic cycles from french guiana. Exp. Parasitol., 81, 20-28 – reference: 13) Mimori, T., Maldonado, M., Samudio, M., Rojas de Arias, A., Moreno, R. and Sakamoto, M. (1992) : Characterization of Trypanosoma cruzi isolates from Paraguay, using restriction enzyme analysis of kinetoplast DNA. Annal. Trop. Med. Parasitol., 86,231-237 – reference: 4) Canese, A., Adorno, A., Cattoni, A., De Vargas, H., Galeano, A. and Sanchez, A. (1972) : Alteraciones electrocardiogrficas en pacientes con immunofluorescencia positiva para in enfermedad le Chages. Rev. Paraguaya de Microbiol., 7, 15-16 – reference: 8) Gomes, M.L., Macedo, A.M., Pena, S.D. and Chiari, E. (1998) : Genetic relationships between Trypanosoma cruzi strains isolated from chronic chagasic patients in southern Brazil as revealed by RAPD and SSR-PCR analysis. Acta Trop., 69, 99-109 – reference: 14) Mimori, T., Sasaki, J., Nakata, M., Gomez, E.A., Uezato, H., Nonaka, S., Hashiguchi, Y., Furuya, M. and Saya, H. (1998) : Rapid identification of Leishmania species from formalin-fixed biopsy samples by polymorphism-specific polymerase chain reaction. Gene, 210,179-186 – reference: 5) Carreno, H., Rojas, C., Aguilera, X., Apt, W., Miles, M. and Solari, A. (1987) : Schizodeme analysis of Trypanosoma cruzi zymodemes from Chile. Exp. Parasitol., 64,252-260 – reference: 7) Ebert, F. (1982) : The identification of two maingroups of Trypanosoma cruzi stocks from Brazil by their isoenzyme patterns of isoelectrofocusing. Tropenmed. Parasitol., 33,140-146 – reference: 9) Higo, H., Yanagi, T., Matta, V., Agatsuma, T., Kanbara, H., Tada, I., De Leon, M.P., Monroy, C. and Tabaru, Y. (1997) : Genetic Structure of Trypanosoma cruzi in Central America and its comparison with South America strains. Inter. J. Parasitol., 27, 1369-1374 – reference: 12) Miles, M.A. and Cibulskis, R.E. (1986) : The heterogeneity of Trypanosoma cruzi zymodeme characterization of Trypanosoma cruzi. Parasitol. Today, 2, 94-97 – reference: 16) Steindel, M., Neto, E.D., De Menezes, C.L.P., Romanha, A.J. and Simpson, A.J.G. (1993) : Random amplified polymorphic DNA analysis of Trypanosoma cruzi strains. Mol. Biochem. Parasitol., 60, 71-80 – reference: 3) Canese, A. (1978) : Datos actualizados soble conocimientos epidemiologicos de la enfermedad de Chagas en el Paraguay. Revista Paraguaya de Microbiol., 13, 3-6 – reference: 19) Vidigal, T.H.D.A., Neto, E.D., Carvalho, O.D.S.C. and Simpson, A.J.G. (1994) : Biomphalaria glabrata : Extensive genetic variation in Brazillian isolates revealed by random amplified polymorphic DNA analysis. Exp. Parasitol., 79,187-194 |
| SSID | ssj0003219810 |
| Score | 1.2682253 |
| Snippet | The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of... |
| SourceID | unpaywall crossref jstage |
| SourceType | Open Access Repository Index Database Publisher |
| StartPage | 427 |
| SubjectTerms | Chagas' disease Paraguay random amplified polymorphic DNA (RAPD) Trypanosoma cruzi unweighted pair group method analysis (UPGMA) |
| Title | COMPARATIVE STUDY OF RANDOM AMPLIFIED POLYMORPHIC DNA OF TRYPANOSOMA CRUZI ISOLATES FROM PARAGUAY |
| URI | https://www.jstage.jst.go.jp/article/tmh1973/27/3/27_3_427/_article/-char/en https://www.jstage.jst.go.jp/article/tmh1973/27/3/27_3_427/_pdf |
| UnpaywallVersion | publishedVersion |
| Volume | 27 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Japanese Journal of Tropical Medicine and Hygiene, 1999/09/15, Vol.27(3), pp.427-431 |
| journalDatabaseRights | – providerCode: PRVAFT databaseName: Open Access Digital Library customDbUrl: eissn: 2186-1811 dateEnd: 20031231 omitProxy: true ssIdentifier: ssj0003219810 issn: 0304-2146 databaseCode: KQ8 dateStart: 19730101 isFulltext: true titleUrlDefault: http://grweb.coalliance.org/oadl/oadl.html providerName: Colorado Alliance of Research Libraries |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3db9MwED-xDgF74BtRBpMfkOAlaWo7TiLxMk2bJtAmkKg0noLt2KWjTaIuEer-es6zW6q9gMSLHSUnx7mz7iP2_Q7gbe62ctDwRiKrtAPVZk4PojLMbW6opVRZl5x8di5OJ_zjRXoR6pxehWOVl-gXTY3r4mkTX7ajwMRRt_gxLjI2wnDdNSUrOV6WbWV3YFekGD4PYHdy_vnwm9874BH1-UWu8FKEtmzsYRvxbrEeLKZZzF1NmS2zdNfPYA_u93UrV7_kfL5ldE4ewff1dP1Zk59x36lYX99CcvyP73kMD4NDSg498RO4Y-qncO8sbLk_A3n0Bx-c3IDRksYStHBVsyDSnUe36MWStpmvFg0KbaZJVUtH0y1XqGqaq2YhiV721zMyw4XufFviklqIG3Xay9VzmJwcfz06jUJdhkgzdDcidJrQkRGJFCKpCpvLjKe5EtwIOjaaJ9grllDDhCpu0F1MYllhMRZhVlKp2AsY1E1tXgKhOiuEVkIKNJPcpEpTxkyaGclzabUYwru1WMrWw2-UGLY4AZaBeyUyDtk2hA-ezxu6wOVtutAg-eapS25DDTGE9xtZ_-VNr_6ddB8eeJgH98vmNQy6ZW_eoBPTqQPY-fQlPwjL9TcfdvAr |
| linkProvider | Unpaywall |
| linkToUnpaywall | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV3Nb9MwFH8aHQJ2GN-iMJAPSHBJmtqOk0hcpolpmrSJA5XGKdiOXTraJOoSoe6v53l2u4oLk3axo-TJcd6z3kfs93sAH3O3lYOGNxJZpR2oNnN6EJVhbnNDLaXKuuTks3NxMuGnF-lFqHN6FY5VXqJfNDWui6dNfNmOAhNH3eLXuMjYCMN115Ss5HhZtpV9ALsixfB5ALuT82-HP_zeAY-ozy9yhZcitGVjD9uId4v1YDHNYu5qymyZpYd-BnvwuK9bufoj5_Mto3P8FH6up-vPmvyO-07F-vofJMd7fM8z2A8OKTn0xM9hx9Qv4NFZ2HJ_CfLoFh-c3IDRksYStHBVsyDSnUe36MWStpmvFg0KbaZJVUtH0y1XqGqaq2YhiV721zMyw4XufFviklqIG3Xay9UrmBx__X50EoW6DJFm6G5E6DShIyMSKURSFTaXGU9zJbgRdGw0T7BXLKGGCVXcoLuYxLLCYizCrKRSsdcwqJvavAFCdVYIrYQUaCa5SZWmjJk0M5Ln0moxhE9rsZSth98oMWxxAiwD90pkHLJtCF88nzd0gcvbdKFB8s1Tl9yGGmIInzey_s-b3t6d9B088TAP7pfNAQy6ZW_eoxPTqQ9hof4Fg9XvNg |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=COMPARATIVE+STUDY+OF+RANDOM+AMPLIFIED+POLYMORPHIC+DNA+OF+TRYPANOSOMA+CRUZI+ISOLATES+FROM+PARAGUAY&rft.jtitle=Japanese+Journal+of+Tropical+Medicine+and+Hygiene&rft.au=MIMORI%2C+TATSUYUKI&rft.au=MALDONADO%2C+MARISEL&rft.au=BABA%2C+SAYUMI&rft.au=SAKAMOTO%2C+MAKOTO&rft.date=1999&rft.pub=Japanese+Society+of+Tropical+Medicine&rft.issn=0304-2146&rft.eissn=2186-1811&rft.volume=27&rft.issue=3&rft.spage=427&rft.epage=431&rft_id=info:doi/10.2149%2Ftmh1973.27.427&rft.externalDocID=article_tmh1973_27_3_27_3_427_article_char_en |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0304-2146&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0304-2146&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0304-2146&client=summon |