COMPARATIVE STUDY OF RANDOM AMPLIFIED POLYMORPHIC DNA OF TRYPANOSOMA CRUZI ISOLATES FROM PARAGUAY

The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established st...

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Published inJapanese Journal of Tropical Medicine and Hygiene Vol. 27; no. 3; pp. 427 - 431
Main Authors MIMORI, TATSUYUKI, MALDONADO, MARISEL, BABA, SAYUMI, SAKAMOTO, MAKOTO, SAYA, HIDEYUKI, MATSUMOTO, TAMAMI, ARIAS, ANTONIETA ROJAS DE, KANBARA, HIROJI
Format Journal Article
LanguageEnglish
Published Japanese Society of Tropical Medicine 1999
Subjects
Chagas' disease
Paraguay
random amplified polymorphic DNA (RAPD)
Trypanosoma cruzi
unweighted pair group method analysis (UPGMA)
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ISSN0304-2146
2186-1811
2186-1811
DOI10.2149/tmh1973.27.427

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Summary:The variation of Paraguayan Trypanosoma cruzi isolates was examined using the unweighted pair group method analysis (UPGMA) based on the resulting matrix of the Dice similarity coefficients of random amplified polymorphic DNA (RAPD) profiles. These isolates were also compared with the established strains isolated from Brazil, Chile and Colombia in South America. Of 9 Paraguayan isolates, 3 isolates were similar to the Berenice, Y and Sao Felipe strains (zymodeme type; Brazilian Z2), respectively, isolated from Brazil, and 4 strains were similar to the Tulahuen strain from Chile. There was no isolate similar to the Colombiana I strain (Z1) from Colombia.
ISSN:0304-2146
2186-1811
2186-1811
DOI:10.2149/tmh1973.27.427