INDUCTION OF NEPHROSIS BY β-CYCLODEXTRIN IN BEAGLES

• Published in: Journal of Toxicologic Pathology Vol. 4; no. 1; pp. 67 - 73
• Main Authors: Okajima, Eigoro, Yamaguchi, Hisako, Kitahori, Yoshiteru, Hiasa, Yoshio, Matsuki, Hisashi, Hirao, Yoshihiko, Uemura, Hirotsugu, Samma, Shoji, Ozono, Seiichiro, Kitagawa, Hisayo, Tabata, Shoichi
• Format: Journal Article
• Language: English
• Published: JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY 1991
• Subjects: Dogs; Histochemistry; Toxic nephrosis; β-cyclodextrin
• ISSN: 0914-9198; 1881-915X
• DOI: 10.1293/tox.4.67

The present study was conducted to examine nephrotoxicity of β-cyclodextrin (β-C) in dogs. Five female Beagle dogs were divided into 3 groups: Group 1 (2 dogs treated with 0.9g/kg of β-C), Group 2 (2 dogs treated with 0.45g/kg of β-C), and Group 3 (1 dog treated with saline). Durations of β-C injection were 7 and 10 consecutive days in Groups 1 and 2. Histopathological and histo-chemical examinations of the kidney were performed. Following 0.9g/kg or long-term 0.45g/kg treatment with β-C, the animals showed marked fatigue and elevated BUN and Cr. Histopathologically, β-C treatment resulted in vacuolation, necrosis, and regeneration in the proximal tubules. Activities of succinic dehydrogenase, β-glucuronidase, and glucose-6-phosphatase decreased histochemically after β-C treatment. Because signs of nephrotoxicity represented were noted in dogs after β-C treatment, as has been reported in rats, it seems likely that dogs also can serve as a model of nephrosis. If this model is to be applied to the study of renal carcinogenesis, the present study suggests the optimum dosing regimen for β-C to be 7-day 0.45g/kg treatment.