Serum oxLDL–β2GPI complex reflects metabolic syndrome and inflammation in adipose tissue in obese

• Published in: International Journal of Obesity Vol. 42; no. 3; pp. 405 - 411
• Main Authors: Siklova, M, Koc, M, Rossmeislová, L, Kraml, P
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2018
• Subjects: 38; 38/90; 631/443/319/1642/393; 631/443/319/2723; 631/45/287/1191; 692/53; Adipose Tissue - metabolism; Adipose Tissue - physiopathology; Adult; beta 2-Glycoprotein I - blood; beta 2-Glycoprotein I - chemistry; beta 2-Glycoprotein I - metabolism; Biomarkers - blood; Cohort Studies; Epidemiology; Female; Health Promotion and Disease Prevention; Humans; Inflammation - blood; Inflammation - physiopathology; Internal Medicine; Lipoproteins, LDL - blood; Lipoproteins, LDL - chemistry; Lipoproteins, LDL - metabolism; Medicine; Medicine & Public Health; Metabolic Diseases; Metabolic Syndrome - blood; Metabolic Syndrome - physiopathology; Middle Aged; Multiprotein Complexes; Obesity - blood; Obesity - metabolism; Obesity - physiopathology; original-article; Public Health
• ISSN: 0307-0565; 1476-5497; 1476-5497
• DOI: 10.1038/ijo.2017.260

Background/Objectives: OxLDL–β2GPI complex has been suggested to have a role in the development of atherosclerosis and other inflammatory diseases. The aim of this study was to investigate the possible association of circulating oxLDL–β2GPI with obesity-induced inflammatory state of adipose tissue and related comorbidities as metabolic syndrome development. Subjects/Methods: Two cohorts of subjects were examined in the study. Cohort I: 36 women with wide range of body mass index (17–48 kg m − 2 ) and metabolic status (with or without metabolic syndrome (MS); cohort II: 20 obese women undergoing a dietary intervention (DI) consisting of 1-month very-low-calorie diet, and 5 months of weight-stabilization period. Serum levels of oxLDL–β2GPI were measured by enzyme-linked immunosorbent assay. Insulin sensitivity was evaluated by hyperinsulinemic-euglycemic clamp and homeostasis model assessment of insulin resistance. mRNA expression of macrophage markers was determined in both subcutaneous (SAT) and visceral (VAT) adipose tissue in cohort I and in SAT in cohort II. Results: Serum oxLDL–β2GPI levels were increased in obese subjects with MS compared to lean or obese without MS (obese with MS: 26.6±5.0 vs lean: 15.17±1.97, P <0.001; vs obese without MS: 16.36±2.89, P <0.05). Serum oxLDL–β2GPI correlated with MS indices (glucose, high-density lipoprotein, triglyceride and ureic acid) and with mRNA expression of macrophage markers in VAT. Weight-reducing DI decreased serum oxLDL–β2GPI levels together with lipid parameters and the mRNA expression of inflammatory markers in SAT. Conclusions: OxLDL–β2GPI seems to be an important marker of visceral adipose tissue inflammation and possibly a factor contributing to insulin resistance and metabolic syndrome development in obese patients.