Protective Effect of Fucoidan Extract from Ecklonia cava on Hydrogen Peroxide-Induced Neurotoxicity

• Published in: Journal of microbiology and biotechnology Vol. 28; no. 1; pp. 40 - 49
• Main Authors: Park, Seon Kyeong, Kang, Jin Yong, Kim, Jong Min, Park, Sang Hyun, Kwon, Bong Seok, Kim, Gun-Hee, Heo, Ho Jin
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.01.2018
• Subjects: Adenosine Triphosphate - analysis; Animals; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cell Extracts - isolation & purification; Cell Extracts - pharmacology; Cell Line; Cell Survival - drug effects; Humans; Hydrogen Peroxide - toxicity; Membrane Potentials - drug effects; Mitochondrial Membranes - drug effects; Mitochondrial Membranes - physiology; Neurons - chemistry; Neurons - drug effects; Neurons - physiology; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; Oxidants - toxicity; Phaeophyceae - chemistry; Polysaccharides - isolation & purification; Polysaccharides - pharmacology; Rats; Reactive Oxygen Species - analysis; 생물학; neuronal cells; Ecklonia cava; fucoidan; mitochondria; oxidative stress
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1710.10043

We evaluated the antioxidant activity and neuronal cell-protective effect of fucoidan extract from (FEC) on hydrogen peroxide (H₂O₂)-induced cytotoxicity in PC-12 and MC-IXC cells to assess its protective effect against oxidative stress. Antioxidant activities were examined using the ABTS radical scavenging activity and malondialdehyde-inhibitory effect, and the results showed that FEC had significant antioxidant activity. Intracellular ROS contents and neuronal cell viability were investigated using the DCF-DA assay and MTT reduction assay. FEC also showed remarkable neuronal cell-protective effect compared with vitamin C as a positive control for both H₂O₂-treated PC-12 and MC-IXC cells. Based on the neuronal cell-protective effects, mitochondrial function was analyzed in PC-12 cells, and FEC significantly restored mitochondrial damage by increasing the mitochondrial membrane potential (Δψm) and ATP levels and regulating mitochondrial-mediated proteins ( -AMPK and BAX). Finally, the inhibitory effects against acetylcholinesterase (AChE), which is a critical hydrolyzing enzyme of the neurotransmitter acetylcholine in the cholinergic system, were investigated (IC₅₀ value = 1.3 mg/ml) and showed a mixed (competitive and noncompetitive) pattern of inhibition. Our findings suggest that FEC may be used as a potential material for alleviating oxidative stress-induced neuronal damage by regulating mitochondrial function and AChE inhibition.