Optimization of Herbicidin A Production in Submerged Culture of Streptomyces scopuliridis M40

Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratio...

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Published inJournal of microbiology and biotechnology Vol. 27; no. 5; pp. 947 - 955
Main Authors Ha, Sanghyun, Lee, Keon Jin, Lee, Sang Il, Gwak, Hyun Jung, Lee, Jong-Hee, Kim, Tae-Woon, Choi, Hak-Jong, Jang, Ja-Young, Choi, Jung-Sub, Kim, Chang-Jin, Kim, Jin-Cheol, Kim, Hyeong Hwan, Park, Hae Woong
Format Journal Article
LanguageEnglish
Published Korea (South) 한국미생물·생명공학회 28.05.2017
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ISSN1017-7825
1738-8872
DOI10.4014/jmb.1611.11005

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Summary:Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to 180 μm, high volumetric production of herbicidin A was obtained with OUR values >137 mg O /l·h. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of 100-200 μm length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
ISSN:1017-7825
1738-8872
DOI:10.4014/jmb.1611.11005