Quantitative Analysis of Serum High Density Lipoprotein by Rocket Immunoelectrophoresis

• Published in: Nihon Rōnen Igakkai zasshi Vol. 16; no. 4; pp. 314 - 319
• Main Authors: Miki, Hideo, Hamada, Noriko, Wada, Kazunari, Kusukawa, Reizo, Okuda, Fumio
• Format: Journal Article
• Language: Japanese
• Published: Japan The Japan Geriatrics Society 1979
• Subjects: Adult; Female; Humans; Immunoelectrophoresis - methods; Lipoproteins, HDL - blood; Male; Middle Aged
• ISSN: 0300-9173
• DOI: 10.3143/geriatrics.16.314

A quantitative analysis of serum high density lipoprotein (HDL) was carried out using rocket immunoelectrophoresis with a commercially prepared anti-HDL-serum as a clinically convenient method. The values obtained from healthy subjects were compared with those from patients with myocardial infarctions. In the Ouchterlony method using the anti-HDL- serum, precipitation lines were detected only against whole serum and HDL (1.21<d<1.063), and these lines were fused. No precipitation lines were detected against VLDL (d<1.006) and LDL (1.063>d>1.006). Under immunoelectrophoresis, two precipitation lines were found between antiserum and human whole serum. One strong arch of precipitations had an electrophoretic mobility corresponding to that of serum-α-globulin, the other was faintly detected in a more cathodal site. The strong arch was shown by the lipid stain Sudan Balack B. With rocket immunoelectrophoresis, only one precipitation line developed in each serum sample agarose gel plate containing anti-HDL-serum, and each of these lines was stained by Sudan Black B. Accordingly, antiserum can be used for the clear determination of serum HDL levels and especially, the apoHDL concentration. Serum HDL levels were determined quantitatively in patients with old myocardial infarctions and healthy subjects. The mean value of serum HDL was significantly lower in the former group than in the latter group. It is interesting to investigate not only the serum HDL-cholesterol levels but also the serum apoHDL values. Especially important is the interrelation between these values in patients with ischemic heart disease. The rocket immunoelectrophoresis is simple and useful method for this purpose.