In vitro Adipocyte Differentiation Inhibition and in vivo Effects on Lipid Metabolism in High-Fat Diet-Induced Obesity of Euphorbia humifusa

• Published in: Journal of microbiology and biotechnology Vol. 34; no. 2; pp. 387 - 398
• Main Authors: Lee, Sung-Gyu, Kang, Hyun
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 01.02.2024
• Subjects: 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; Anti-Obesity Agents - pharmacology; Anti-Obesity Agents - therapeutic use; Cholesterol, LDL; Diet, High-Fat - adverse effects; Euphorbia - metabolism; Lipid Metabolism; Mice; Mice, Inbred C57BL; Mice, Obese; Obesity - drug therapy; Obesity - metabolism; 생물학; Euphorbia humifusa; adipocyte differentiation; SREBP-1c; 3T3-L1; obesity
• ISSN: 1017-7825; 1738-8872; 1738-8872
• DOI: 10.4014/jmb.2308.08004

Willd ( ) is a functional raw material with various pharmacological activities. This study aimed to validate the inhibitory effect of extract (EHE) on adipocyte differentiation in vitro and in a high-fat-diet (HFD)-induced mouse model to evaluate the as a novel anti-obesity and lipid metabolism enhancer agent. EHE effects on obesity and lipid metabolism were assessed in HFD-induced obese mice after 4-week treatments. Results were compared among four treatment groups ( = 7/group): low fat diet (LFD), high fat diet (HFD), and HFD-induced obese mice treated with either 100 or 200 mg/kg/day EHE (EHE100 and EHE200, respectively). EHE (50 to 200 μg/ml) and quercetin (50 μg/ml) significantly reduced 3T3-L1 preadipocyte differentiation ( < 0.001), in a concentration-dependent manner. EHE affected lipid metabolism, as evidenced by changes in serum lipid components. The HFD-EHE100 and HFD-EHE200 groups exhibited significantly ( < 0.05) reduced triglycerides (TG, 97.50 ± 6.56 and 82.50 ± 13.20 mg/dL, respectively) and low-density lipoprotein-cholesterol (LDL-c: 40.25 ± 4.99 and 41.25 ± 6.36 mg/dL, respectively) compared to the HFD group (TG: 129.25 ± 19.81 mg/dL; LDL-c: 51.75 ± 11.59 mg/dL). Haematoxylin and Eosin (H&E) and Oil red O staining showed that EHE markedly reduced lipid accumulation and inhibited lipogenesis in the liver. Interestingly, EHE significantly ( < 0.01) reduced the expression of adipogenic transcription factors in liver tissue. Our results indicated that EHE has the potential to be a therapeutic agent for addressing obesity and lipid metabolism.