ROR2 gene is associated with risk of non-syndromic cleft palate in an Asian population

• Published in: Chinese medical journal Vol. 125; no. 3; pp. 476 - 480
• Main Authors: Wang, Hong, Hetmanski, Jacqueline B, Ruczinski, Ingo, Liang, Kung Yee, Fallin, M Daniele, Redett, Richard J, Raymond, Gerald V, Chou, Yah-Huei Wu, Chen, Philip Kuo-Ting, Yeow, Vincent, Chong, Samuel S, Cheah, Felicia Sh, Jabs, Ethylin Wang, Scott, Alan F, Beaty, Terri H
• Format: Journal Article
• Language: English
• Published: China Mount Sinai School of Medicine, New York City, New York, USA%Department of Medicine , Johns Hopkins University School of Medicine, Baltimore, USA 01.02.2012; Department of Epidemiology and Biostatistics, Peking University School of Public Health, Beijing 100191, China%Department of Epidemiology Johns Hopkins Bloomberg School of Public Health, Baltimore, USA%Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, USA%Department of Life Sciences and Institute of Genome Sciences,National Yang-Ming University, Taipei, Taiwan, China%Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, USA%Kennedy Krieger Institute , Johns Hopkins University School of Medicine, Baltimore, USA%Department of Medical Research,Chang Gung Memorial Hospital, Taoyuan,Taiwan, China%Craniofacial Center, Chang Gung Memorial Hospital, Taoyuan,Taiwan, China%Department of Plastic, Reconstructive & Aesthetic Surgery, KK Women's & Children's Hospital, Singapore%Department of Pediatrics, National University of Singapore,Singapore%Department of Pediatrics , Johns Hopkins University School of Medicine, Baltimore, USA
• Subjects: Asian Continental Ancestry Group - genetics; Cleft Lip - genetics; Cleft Palate - genetics; Genetic Predisposition to Disease - genetics; Genotype; Humans; Linkage Disequilibrium - genetics; Polymorphism, Single Nucleotide - genetics; Receptor Tyrosine Kinase-like Orphan Receptors - genetics; SNPs; 亚洲; 人口; 单核苷酸多态性; 受体酪氨酸激酶; 基因测试; 综合征; 风险; cleft lip; cleft palate; association; transmission disequilibrium test; receptor tyrosine kinase-like orphan receptor 2
• ISSN: 0366-6999; 2542-5641; 2542-5641; 0366-6999
• DOI: 10.3760/cma.j.issn.0366-6999.2012.03.014

Background The receptor tyrosine kinase-like orphan receptor 2 （ROR2） gene has been recently shown to play important roles in palatal development in animal models and resides in the chromosomal region linked to non syndromic cleft lip with or without cleft palate in humans.The aim of this study was to investigate the possible association between ROR2 gene and non-syndromic oral clefts.Methods Here we tested 38 eligible single-nucleotide polymorphisms （SNPs） in ROR2 gene in 297 non-syndromic cleft lip with or without cleft palate and in 82 non-syndromic cleft palate case parent trios recruited from Asia and Maryland.Family Based Association Test was used to test for deviation from Mendelian inheritance.Plink software was used to test potential parent of origin effect.Possible maternally mediated in utero effects were assessed using the TRlad Multi-Marker approach under an assumption of mating symmetry in the population.Results Significant evidence of linkage and association was shown for 3 SNPs （rs7858435,rs10820914 and rs3905385） among 57 Asian non-syndromic cleft palate trios in Family Based Association Tests.P values for these 3 SNPs equaled to 0.000068,0.000115 and 0.000464 respectively which were all less than the significance level （0.05/38=0.0013） adjusted by strict Bonferroni correction.Relevant odds ratios for the risk allele were 3.42 （1.80-6.50）,3.45 （1.75-6.67） and 2.94 （1.56-5.56）,respectively.Statistical evidence of linkage and association was not shown for study groups other than non-syndromic cleft palate.Neither evidence for parent-of-origin nor maternal genotypic effect was shown for any of the ROR2 markers in our analysis for all study groups.Conclusion Our results provided evidence of linkage and association between the ROR2 gene and a gene controlling risk to non-syndromic cleft palate.