Altered Fecal Microbiome Years after Traumatic Brain Injury

• Published in: Journal of neurotrauma Vol. 37; no. 8; pp. 1037 - 1051
• Main Authors: Urban, Randall J., Pyles, Richard B., Stewart, Christopher J., Ajami, Nadim, Randolph, Kathleen M., Durham, William J., Danesi, Christopher P., Dillon, E. Lichar, Summons, Jennifer R., Singh, Charan K., Morrison, Melissa, Kreber, Lisa A., Masel, Brent, Miller, Aaron L., Wright, Traver J., Sheffield-Moore, Melinda
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 15.04.2020
• Subjects: Alanine; Amino acids; Cognition; Cognition & reasoning; Complications; Fecal microflora; Feces; Genomes; Guillain-Barre syndrome; Gut-brain axis; Meals; Metagenomics; Microbiomes; Morbidity; Polymerase chain reaction; rRNA 16S; Sarcosine; Speciation; Therapeutic applications; Trauma; Traumatic brain injury; Tryptophan; Tuf gene; Whole genome sequencing; Bakersfield California; United States > US; moderate-to-severe TBI; microbiome; long-term care
• ISSN: 0897-7151; 1557-9042; 1557-9042
• DOI: 10.1089/neu.2019.6688

Patients with chronic traumatic brain injury (TBI) requiring long-term, permanent care suffer a myriad of clinical symptoms (i.e., impaired cognition, fatigue, and other conditions) that persist for years beyond the acute brain injury. In addition to these comorbid clinical symptoms, chronic TBI patients exhibit altered amino acid and hormonal profiles with distinct cytokine patterns suggesting chronic inflammation. This metabolic link suggests a role of the gut-brain axis in chronic TBI. Thus, we utilized a two-site trial to investigate the role of the gut-brain axis in comorbidities of chronic TBI. The fecal microbiome profile of 22 moderate/severe TBI patients residing in permanent care facilities in Texas and California was compared to 18 healthy age-matched control subjects working within the participating facilities. Each fecal microbiome was characterized by 16S(V4) ribosomal RNA (rRNA) gene sequencing and metagenomic genome sequencing approaches followed by confirmatory full 16S rRNA gene sequencing or focused gene speciation and specific quantitative polymerase chain reaction evaluation of selected genera or species. The average chronic TBI patient fecal microbiome structure was significantly different compared to the control cohort, and these differences persisted after group stratification analysis to identify any unexpected confounders. Notably, the fecal microbiome of the chronic TBI cohort had absent or reduced spp. and spp. Conversely, bacteria in the Ruminococcaceae family were higher in abundance in TBI compared to control profiles. Previously reported hypoaminoacidemia, including significantly reduced levels of l-tryptophan, l-sarcosine, ß-alanine, and alanine, positively correlated with the reduced levels of spp. in the TBI cohort samples compared to controls. Although the sequelae of gut-brain axis disruption after TBI is not fully understood, characterizing TBI-related alterations in the fecal microbiome may provide biomarkers and therapeutic targets to address patient morbidity.