Mathematical analysis of colonial formation of embryonic stem cells in microfluidic system

• Published in: The Korean journal of chemical engineering Vol. 29; no. 3; pp. 392 - 395
• Main Authors: Min, Seul Ki, Lee, Byung Man, Hwang, Jin Ha, Ha, Sung Ho, Shin, Hwa Sung
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2012; 한국화학공학회
• Subjects: Biotechnology; Catalysis; Chemistry; Chemistry and Materials Science; Industrial Chemistry/Chemical Engineering; Materials Science; 화학공학; ); Self-renewal; Embryonic Stem Cells; Free Peripheral Length Ratio (L; Morphology; Perfusion Culture
• ISSN: 0256-1115; 1975-7220
• DOI: 10.1007/s11814-011-0181-7

A fluidic environment affects mechanochemical characteristics of embryonic stem cells (ESCs). Perfusion is recognized as an attractive culture mode of ESCs since the steady fluidic state can enhance ESCs’ controllability, supporting a unique cell culture condition. Cellular membrane motility presents important information about cellular dynamics such as adhesion, spreading, and migration. Thus, an investigation of the perfusion-induced membrane motility is significant to understand the mechanochemical behavior of ESCs in the steady culture state. In this research, we suggest L fr , the ratio of circumferential membrane unattached to other cells’ to the cell’s circumference, as a new parameter to characterize cells’ shape and motility. L fr of embryonic stem cells has positive correlations with cellular area (A r ) and free peripheral length (L f ) but a negative correlation with roundness (R n ). We also propose a mathematical model representing ESCs’ membrane motilities and demonstrate their colonical behavior.