The point mutation induced by the low-energy N+ ion implantation in Impatiens balsamine genome

• Published in: Russian journal of genetics Vol. 48; no. 10; pp. 1009 - 1014
• Main Authors: Gao, W. J., Su, J. X., Xie, L., Deng, C. L., Zhang, T., Lu, L. D.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.10.2012
• Subjects: adenine; Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; cytosine; energy; genome; guanine; Human Genetics; Impatiens; ions; Microbial Genetics and Genomics; mutagenicity; mutants; nucleotide sequences; Plant Genetics; point mutation; random amplified polymorphic DNA technique; sequence analysis; thymine; Wild Type Plant; ISSR Primer; Impatiens Balsamine; Primer Prime Sequence; RAPD Primer
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795412070034

To investigate the effect of genome mutations induced by low energy ions implantation in higher plants, genome mutation of Impatiens balsamine mutant induced by low energy N + ion implantation were analyzed by the RAPD, ISSR and genome sequence. Six out of the 121 ISSR primers and 6 out of the 135 RAPD primers showed that polymorphism ratios between mutants and wild type were 4.96% and 2.89%, respectively. Sequence analysis revealed that base deletions, insertions, and substitutions were observed in the mutant genome comparable to wild type. N+ induced point mutations were mostly base substitution (77.4%), no duplication, long fragments insertions and deletions was found. In all point mutation, adenine (A) was most sensitive to the N + ion implantation in impatiens. The transition was mainly A → guanine (G) (15.90%) and thymine (T) cytosine (C) (12.55%). Transversion happened in A ↔ T (16.74%), which much higher than C ↔ G (5.02%), G ↔ T (6.69%), A ↔ C (7.11%) bases. These findings indicate that low energy ions being a useful mutagen were mostly cause the point mutation in impatiens.