LC–MS/MS Determination of Ginsenoside Compound K and its Metabolite 20( S )-Protopanaxadiol in Human Plasma and Urine: Applications in a Clinical Study

• Published in: Bioanalysis Vol. 11; no. 5; pp. 365 - 380
• Main Authors: Yang, Li, Wang, Chun-Yang, Xie, Xiao-Nv, Wang, Yi-Cheng, Peng, Jing-Bo, Huang, Wei-Hua, Chen, Yao, Ouyang, Dong-Sheng, Tan, Zhi-Rong
• Format: Journal Article
• Language: English
• Published: England Newlands Press 01.03.2019
• Subjects: Acids; Anti-inflammatory agents; Arthritis, Rheumatoid; Biological products; Chromatography; Chromatography, Liquid - methods; Female; Ginsenosides; Ginsenosides - pharmacology; Ginsenosides - therapeutic use; Humans; Inflammation; Ions; Male; Mass spectrometry; Mass spectroscopy; Metabolites; Microorganisms; Panax - chemistry; Pharmaceuticals; Plasma; Rheumatoid arthritis; Sapogenins - pharmacology; Sapogenins - therapeutic use; Scientific imaging; Studies; Tandem Mass Spectrometry - methods; Urine; Beijing China; United States > US; China; Germany; ginsenoside compound K; pharmacokinetics; LC–MS/MS; 20-protopanaxadiol
• ISSN: 1757-6180; 1757-6199
• DOI: 10.4155/bio-2018-0185

Ginsenoside compound K (CK) is considered to be a potential therapeutic drug for rheumatoid arthritis because of its good anti-inflammatory activity. The purpose of this work was to establish a rapid, sensitive and specific method for determination of CK and its active metabolite 20(S)-protopanaxadiol (20(S)-PPD). Materials & methods: The analytes and internal standards were extracted by liquid-liquid extraction. Then, were separated by high performance liquid phase and determined by triple quadrupole mass spectrometry. A LC-MS/MS using liquid-liquid extraction was developed for determining CK over the concentration range 1.00-1002.00 ng/ml and 0.15-54.30 ng/ml for 20(S)-PPD. The lower limits of quantification for CK and 20(S)-PPD were 1.00 and 0.15 ng/ml, respectively. This method was successfully validated for detecting both CK and 20(S)-PPD in the human plasma and urine, and was proved to be suitable for the pharmacokinetic study of CK in healthy Chinese volunteers. ChiCTR-TRC-14004824.