Combined effect of temperature and water stress on seed germination of four Leptocereus spp. (Cactaceae) from Cuban dry forests

• Published in: Plant species biology Vol. 36; no. 3; pp. 512 - 522
• Main Authors: Barrios, Duniel, Flores, Joel, Sánchez, Jorge A, González‐Torres, Luis R
• Format: Journal Article
• Language: English
• Published: Melbourne John Wiley & Sons Australia, Ltd 01.07.2021
• Subjects: aquifers; Cactaceae; cacti and succulents; Caribbean; coasts; Cuba; high temperature; rain; seed germination; seed recovery; seed weight; seedling performance; seedlings; seeds; soil water; temperature; water potential; water stress; Caribbean; Cuba
• ISSN: 0913-557X; 1442-1984
• DOI: 10.1111/1442-1984.12334

Cactus seeds have developed adaptations to survive with high temperatures and low soil moisture in their habitats. We studied the effect of the combination of four water potentials (0, −0.2, −0.4 and −0.6 MPa) and two temperatures (25°C and 35°C) on germination and seedling mass of four endemic Leptocereus species from Cuba. There were two semi‐arid coast species (L. arboreus and L. santamarinae) and two species inhabiting dry inland karstic hills (L. ekmanii and L. scopulophilus). We hypothesized that: (a) a decrease in water potential and an increase in temperature would result in low and slow germination, as well as in low seedling mass, and (b) ungerminated cactus seeds after exposure to combined water and temperature stress would have a high recovery capacity. The minimum time required for the seeds to start germination (Tmin), mean germination time (MGT) and germinability were evaluated. In addition, seed mass, the recovery after treatments of non‐germinated seeds, and the seedling fresh mass obtained under different treatments were compared between species. In general, germination was only obtained at 25°C and germinability and seedling mass were drastically affected by the reduction from 0 MPa to −0.2 MPa. Seeds showed thermoinhibition at 35°C at all water potentials. There was a tendency to increase the MGT with decreasing water potential in three species. Low seed recovery occurred at all combined treatments for three species. If the predictions of increased temperature and decreased rainfall for the Caribbean region occur, a reduction in the germination of the Leptocereus species studied is expected. Germination of Leptocereus spp. was only obtained at 25°C and germinability and seedling mass were drastically affected by the reduction from 0 MPa to −0.2 MPa. Seeds showed thermoinhibition at 35°C at all water potentials. Low seed recovery occurred at all combined treatments for three species.