Evaluation of microRNA-146a expression in acute lymphoblastic leukemia

MicroRNAs (miRNAs) play an essential role in the development and progression of acute lymphoblastic leukemia (ALL), and could serve as disease biomarkers and therapeutic targets. The function of miR-146a in lymphoid differentiation has been here with discussed. However, the role of this miRNA in the...

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Published inFrontiers in biology Vol. 11; no. 1; pp. 53 - 58
Main Authors Tavakoli, Farzaneh, Kaveh Jaseb, Mohammad Ali Jalali Far, Masoud Soleimani, Elahe Khodadi, Najmaldin Saki
Format Journal Article
LanguageEnglish
Published Beijing Higher Education Press 01.02.2016
Subjects
Biochemistry
biomarkers
Biomedical and Life Sciences
Cell Biology
Developmental Biology
genes
Genetics and Population Dynamics
leukocytes
Life Sciences
lymphocytic leukemia
microRNA
miRNAs
Neurobiology
patients
prediction
Proteomics
quantitative polymerase chain reaction
Research Article
transcription factors
实时定量PCR
急性
淋巴细胞白血病
生物标记物
融合基因
血小板计数
评价
acute lymphoblastic leukemia
miR-146a
prognostic marker
Online AccessGet full text
ISSN1674-7984
1674-7992
DOI10.1007/s11515-016-1387-1

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Summary:MicroRNAs (miRNAs) play an essential role in the development and progression of acute lymphoblastic leukemia (ALL), and could serve as disease biomarkers and therapeutic targets. The function of miR-146a in lymphoid differentiation has been here with discussed. However, the role of this miRNA in the outcome of ALL is not well understood. Peripheral blood of 48 patients with ALL and 20 age- and sex-matched healthy control subjects was used to accurately evaluate the expression of miR-146a by stem-loop Real time PCR. No statistically significant difference was found between patients and controls in total miR-146a expression. The expression of miR-146a was high (18.75%), low (27.08%) and not different (54.17%) in ALL patients. Our analysis indicated no association between the expression of miR-146a and any prognostic factors such as WBC/PLT counts, Hb, fusion genes (P190 and some translocations) with ALL type. This study revealed that miR-146a cannot be an independent factor for predicting the outcome of ALL patients.We suggest a multi-parameter analysis including miRNAs, transcription factors and critical genes to achieve a precise clinical panel for prognostic values.
Bibliography:11-5892/Q
miR-146a, acute lymphoblastic leukemia, prognostic marker
MicroRNAs （miRNAs） play an essential role in the development and progression of acute lymphoblastic leukemia （ALL）, and could serve as disease biomarkers and therapeutic targets. The function of miR-146a in lymphoid differentiation has been here with discussed. However, the role of this miRNA in the outcome of ALL is not well understood. Peripheral blood of 48 patients with ALL and 20 age- and sex-matched healthy control subjects was used to accurately evaluate the expression of miR-146a by stem-loop Real time PCR. No statistically significant difference was found between patients and controls in total miR-146a expression. The expression of miR-146a was high （18.75%）, low （27.08~o） and not different （54.17%） in ALL patients. Our analysis indicated no association between the expression of miR-146a and any prognostic factors such as WBC/PLT counts, Hb, fusion genes （P190 and some translocations） with ALL type. This study revealed that miR-146a cannot be an independent factor for predicting the outcome of ALL patients. We suggest a multi-parameter analysis including miRNAs, transcription factors and critical genes to achieve a precise clinical panel for prognostic values.
http://dx.doi.org/10.1007/s11515-016-1387-1
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ISSN:1674-7984
1674-7992
DOI:10.1007/s11515-016-1387-1