Modeling, optimization, and characterization of polysaccharides from Strychnos potatorum using microwave-assisted extraction

• Published in: Biomass conversion and biorefinery Vol. 15; no. 2; pp. 2111 - 2129
• Main Authors: Rahman, Sameeha Syed Abdul, Pasupathi, Saroja, Venkatachalam, Ponnusami, Jothi, Arunachalam, Karuppiah, Sugumaran
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2025; Springer Nature B.V
• Subjects: Antioxidants; Artificial neural networks; Biomass; Biorefineries; Biotechnology; Energy; Optimization; Original Article; Polysaccharides; Pseudoplasticity; Renewable and Green Energy; Response surface methodology; Rheological properties; Rheology; Thermodynamic properties; Microwave-assisted extraction; Response surface methodology; Artificial neural network
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-023-04321-7

The polysaccharides extracted from Strychnos potatorum (SPS) have been applied as an effective pharmacological agent for their anti-diabetic activity, anti-inflammatory effect, and hepato-protective and antioxidant activity. Due to the market demand for peculiar characteristics of these polysaccharides, it has gained greater importance to extract a higher yield of polysaccharides. The present study is focused on the application of response surface methodology (RSM) and artificial neural network (ANN) for the optimization of recovery of polysaccharides from SPS through microwave-assisted extraction (MAE). For RSM, three parameters namely SPS concentration (40 g/L), potassium nitrate concentration (1.5 M), and extraction time (13 min) were considered to produce a maximum yield of polysaccharides (0.88 ± 0.02 g/g). The organoleptic , structural, and thermal properties of extracted polysaccharides through CE and MAE (before and after optimization) were examined by various structural and functional analyses to explore the presence of galactomannans and galactans in the extracted polysaccharides. Rheology study has suggested pseudoplastic nature for the extracted polysaccharides from SPS with a molecular mass of sample ranging from 0.5 to 850 kDa. The extracted polysaccharides also showed moderate properties of antioxidant activity (39–41%), water-holding capacity (0.2–1.2), and emulsifying activity (44–48%). These results prove that MAE could be a valid approach to extracting polysaccharides with high molecular weight and moderate antioxidant activity from SPS.