Role of Glutathione-S-Transferase Polymorphism on Arsenic-Induced Protein Carbonylation and Urinary Deoxy Guanosine Status

• Published in: Exposure and health Vol. 12; no. 3; pp. 539 - 546
• Main Authors: Chanda, Sarmishtha, Roy, Jayashree, Guha Mazumder, Debendranath, Chakraborty, Tushar
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.09.2020; Springer Nature B.V
• Subjects: 8-Hydroxydeoxyguanosine; Aquatic Pollution; Arsenic; Carbonyls; Creatinine; DNA damage; DNA methylation; Drinking water; Earth and Environmental Science; Environment; Environmental Health; Enzymes; Exposure; Gene polymorphism; Glutathione; Glutathione transferase; GSTM1 protein; GSTT1 protein; Laboratory animals; Metabolism; Nonparametric statistics; Original Paper; Oxidation; Oxidative stress; Pollution; Polymorphism; Proteins; Urine; Waste Water Technology; Water and Health; Water Management; Water Pollution Control; Water Quality/Water Pollution; West Bengal India; United States > US; Baltimore Maryland; India; Protein carbonylation; GST polymorphism; Arsenic; Oxidative DNA damage
• ISSN: 2451-9766; 2451-9685
• DOI: 10.1007/s12403-019-00319-7

Oxidative stress is significantly associated with chronic arsenic exposure in persons exposed to moderate-to-high level of environmental arsenic. However, the response varies widely among persons within similar exposure level. To study whether Glutathione-S-Transferase (GST) gene polymorphism plays any pivotal role in this variation, total number of 115 chronic arsenic-exposed study subjects without arsenic-induced cancer from School of Tropical Medicine, Kolkata and from arsenic-exposed districts of West Bengal were recruited for this study. Concentration of arsenic in their urine and water, extent of clinical manifestation, GST status and protein carbonylation, and 8-oxo-2′-deoxyguanosine status were determined. Genetic polymorphism of GSTM1 and T1 were significantly associated ( p  < 0.05) with degree of protein carbonylation and 8-oxo-2′-deoxyguanosine in moderate-to-high degree of arsenic exposure groups. Persons having null genotype have significantly increased level of protein carbonylation and 8-oxo-2′-deoxyguanosine in comparison to persons with GSTM1 or GSTT1 non-null genotype of the same arsenic exposure group. Degree of protein carbonylation and urinary 8-oxo-2-deoxyguanosine is positively correlated with GST null genotype.