Isolation and identification of Rhodosporidium diobovatum DS-0205 from deep-sea sediment of eastern Pacific Ocean

• Published in: Journal of Central South University of Technology. Science & technology of mining and metallurgy Vol. 16; no. 6; pp. 942 - 947
• Main Authors: Zeng, Le-ping, Huang, Ju-fang, Qiu, Guan-zhou, Chu, Feng-you, Chen, Dan, Tong, Jian-bin, Luo, Xue-gang
• Format: Journal Article
• Language: English
• Published: Changsha Central South University 01.12.2009
• Subjects: Engineering; Metallic Materials; deep-sea sediment; isolation; identification; ITS and 5.8S rRNA sequences; 18S rRNA
• ISSN: 1005-9784; 1993-0666
• DOI: 10.1007/s11771-009-0157-5

A facultative heterotrophic strain (DS-0205) was isolated from a deep-sea sediment sample collected at a depth of 5.2 km in the eastern Pacific Ocean, China. Strain DS-0205 is motile helmet-like single cell or pairs, forming hemisphere with the center sunken of variable size. It has a widespread carbon source and nitrogen source, including agarose, citric acid, salicin, D -glucitol nitrate, sodium nitrite and ethylamine. It can grow in the following environment: temperature 4–37 °C, pH 2.0–12.0, tolerance to NaCl⩽15%. Two phylogenetic trees, one based on the ITS and 5.8S rRNA sequences and the other based on the 18S rRNA sequences, unite strain DS-0205(=JCM 0205) to the type strain of Rhodosporidium diobovatum JCM 3787 through a considerable evolutionary distance. These results suggest that strain DS-0205 is a new strain of the Rhodosporidium diobovatum .