Phylogenetic and Molecular Analysis of an H7N7 Avian Influenza Virus Isolated in East Dongting Lake in 2012

• Published in: Biomedical and environmental sciences Vol. 28; no. 7; pp. 518 - 526
• Main Authors: YAO, Yi, XU, Cui Ling, SHI, Jing Hong, ZHU, Yun, LI, Yun Fei, BAI, Tian, LI, Fang Cai, CAI, Tao, YUAN, Fan, CHEN, Tao, YANG, Hao, LI, Wen Chao, ZHANG, Heng Jiao, ZHANG, Hong, SHU, Yue Long
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2015; Hunan East Dongting Lake National Nature Reserve, Yueyang 414018, Hunan, China%National Institute for Viral Disease Control and Prevention, China CDC, Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China%Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, Beijing 100045, China%Hunan Provincial Center for Disease Control and Prevention, Changsha 410005, Hunan, China%National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 100050, China
• Subjects: Amino Acid Sequence; Animals; Avian influenza viruses; China; East Dongting Lake wetland; Embryo, Nonmammalian - virology; Feces - virology; Geese - virology; Genome, Viral; H7N7 subtype; Influenza A Virus, H7N7 Subtype - genetics; Influenza A Virus, H7N7 Subtype - isolation & purification; Influenza in Birds - virology; Lakes - virology; Molecular Sequence Data; Phylogeny; Poultry Diseases - virology; Real-Time Polymerase Chain Reaction - veterinary; RNA, Viral - genetics; RT-PCR检测; Wild geese; 东洞庭湖; 低致病性禽流感病毒; 全基因组测序; 分子分析; 分子生物学特性; 环境样品; 系统发育; China; East Dongting Lake wetland; H7N7 subtype; Avian influenza viruses; Wild geese
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2015.074

Objective In March 2012, an H7N7 subtype avian influenza virus （AIV） named A/wild goose/Dongting/PC0360/2022 （H7N7） （DT/PC0360） was recovered from a wild goose in East Dongting Lake. We performed whole-genome sequencing of the isolate, and analyzed the phylogenetic and molecular characterization. Methods RNA was extracted from environment samples （including fecal samples from wild bird or domestic ducks, and water samples） for detecting the presence of Influenza A Virus targeting Matrix gene, using realtime RT-PCR assay. The positive samples were performed virus isolation with embryonated eggs. The subtype of the isolates were identified by RT-PCR assay with the HI-HI6 and N1-N9 primer set. The whole-genome sequencing of isolates were performed. Phylogenetic and molecular characterizations of the eight genes of the isolates were analyzed. Results Our results suggested that all the eight gene segments of DT/PC0360 belonged to the Eurasian gene pool, and the HA gene were belonged to distinct sublineage with H7N9 AIV which caused outbreaks in Mainland China in 2013. The hemagglutinin cleavage site of HA of DT/PC0360 showed characterization of low pathogenic avian influenza virus. Conclusion Strengthening the surveillance of AlVs of wild waterfowl and poultry in this region is vita for our knowledge of the ecology and mechanism of transmission to prevent an influenza pandemic.