Murine IgE Antibody to Recognize Human Major Allergen, Mal f4

• Published in: Nihon Ishinkin Gakkai zasshi Vol. 42; no. 4; pp. 214 - 216
• Main Authors: Murayama, Somey Y., Ono, Yasuo, Wada, Kayoko, Ikeda, Tatsuo, Tansho, Shigeru, Yamaguchi, Hideyo, Mitsuya, Masayasu, Suegara, Nobuo
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Japanese Society for Medical Mycology 2001
• Subjects: Allergens - immunology; Animals; Antibodies, Fungal - immunology; Antibody Specificity; Antigens, Plant; atopic dermatitis; Dermatitis, Atopic - immunology; Dermatitis, Atopic - microbiology; ELISA; Enzyme-Linked Immunosorbent Assay; Female; Humans; IgE; immunoblot; Immunoblotting; Immunoglobulin E - immunology; Malassezia; Malassezia - immunology; Mice; Mice, Inbred BALB C
• ISSN: 0916-4804; 1882-0476
• DOI: 10.3314/jjmm.42.214

To elucidate the specificity of murine IgE antibody against Malassezia furfur, the immunoblot patterns of IgE in sera obtained from mice inoculated repeatedly in the nasal cavity with M. f. cells, or injected intraperitoneally with M. f. cells mixed with Al(OH)3 gel were compared with those of IgE antibody detected in sera from patients with AD. Most of the murine IgE anti-Malassezia antibodies shared some antigenic bands with IgE in sera from patients with AD. The IgE antibody of murine also recognized Mal f4, one of the major allergens detected in patients with AD.