Simultaneous and rapid screening and determination of twelve azo dyes illegally added into food products by using chemometrics-assisted HPLC-DAD strategy

• Published in: Microchemical journal Vol. 171; p. 106775
• Main Authors: Dong, Ming-Yue, Wu, Hai-Long, Long, Wan-Jun, Wang, Tong, Yu, Ru-Qin
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2021
• Subjects: Azo dyes; Chemometrics; High-performance liquid chromatography; Illegal additives; SVD; SIV; PR; MR; High-performance liquid chromatography; Azo dyes; SB; SIII; SII; Illegal additives; SG; ATLD; DY; SI; BY; CORCONDIA; MCR-ALS; TR; Chemometrics; S7B
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/j.microc.2021.106775

[Display omitted] •Chemometrics-assisted HPLC-DAD for screening and determination of twelve azo dyes has been proposed.•Twelve azo dyes can be successfully eluted within 6.5 min.•The proposed strategy can simultaneously detect analytes in different food products.•Both ATLD and MCR-ALS have the advantage of “mathematical separation”. Edible colorants, as an essential external condition of food products, can increase people’s appetite. Some businesses have used some cheap and illegal dyes instead of edible colorants to gain benefits. It not only deceives consumers, but also seriously endangers their health. In this work, two chemometric algorithms assisted high-performance liquid chromatography with diode array detection (HPLC-DAD) were proposed for screening and quantifying twelve azo dyes illegally added into different food products. All analytes were successfully eluted within 6.5 min. With the aid of the prominent “second-order advantage” of the chemometric algorithms, the overlapping peaks and unknown interferences were solved. The spiked average recoveries obtained by alternating trilinear decomposition (ATLD) and multivariate curve resolution-alternating least squares (MCR-ALS) were 82.8–111.5% with standard deviation (SD) less than 6.5% and 78.7–122.6% with SD less than 18.7%, respectively. The limits of detection were 0.01–2.56 mg kg−1 and 0.01–2.95 mg kg−1, respectively. The results indicated that two algorithms had practical value for the simultaneous and rapid screening and determination of azo dyes in food products. Compared with other methods based on HPLC, the proposed method could flexibly handle different interferences, and had the advantages of high efficiency, simple operation and being environmentally friendly.