Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 59; no. 5; pp. 200 - 205
• Main Authors: Hirakawa, Yuki, Iwasa, Seiji, Watanabe, Takaho, Sato, Natsuki, Narita, Hiroshi, Miyake, Shiro, Yamasaki, Tomomi
• Format: Journal Article
• Language: English
• Published: Tokyo Japanese Society for Food Hygiene and Safety 25.10.2018; Japan Science and Technology Agency
• Subjects: aflatoxin; Aflatoxins; ELISA; Food analysis; immunoassay; Monoclonal antibodies; monoclonal antibody; Peanuts; Performance assessment; total aflatoxin
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.59.200

A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50–230 pg/mL for AFB1, 50–270 pg/mL for AFB2, 60–390 pg/mL for AFG1 and 65–700 pg/mL for AFG2. The recovery of AFs from spiked roasted peanuts was 98％. Further, when 4 samples actually contaminated with AFB1, AFB2, AFG1 and AFG2 were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 μg/kg for all foods) in Japan.