Effect of Cold Plasma on Cell Viability and Collagen Synthesis in Cultured Murine Fibroblasts

• Published in: Plasma science & technology Vol. 18; no. 4; pp. 353 - 359
• Main Author: 石兴民 蔡晶芬 许桂敏 任鸿斌 陈思乐 常正实 刘进仁 黄崇亚 张冠军 吴喜利
• Format: Journal Article
• Language: English
• Published: 01.04.2016
• Subjects: Cold plasmas; Collagens; cyclin; Exploration; Fibroblasts; Gene expression; p27基因; Progressions; Synthesis; Viability; 冷等离子体; 小鼠; 成纤维细胞; 细胞活力; 胶原合成
• ISSN: 1009-0630
• DOI: 10.1088/1009-0630/18/4/04

An argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro.Experimental results showed that,compared with the control cells,the treatment of fibroblasts with 15 s of plasma led to a significant increase of cell viability and collagen synthesis,while the treatment of 25 s plasma resulted in a remarkable decrease.Exploration of related mechanisms suggested that cold plasma could up-regulate Cyclin D1 gene expression and down-regulate p27 gene expression at a low dose,while it could down-regulate Cyclin D1 expression and up-regulate p27 expression at a higher dose,thus altering the cell cycle progression,and then affecting cell viability and collagen synthesis of fibroblasts.