Sex difference in liver regeneration and ornithine decarboxylase protein after acute ethanol administration to hepatectomized rats

• Published in: Kanzo Vol. 31; no. 6; pp. 610 - 616
• Main Authors: HIRAKAWA, Jyunichi, OOHATA, Mitsuru, NAKAJIMA, Hisato, NAKAHARA, Masao, FUJISAWA, Kiyoshi, KITAHARA, Toshihisa, KAMEDA, Haruo, YAMAUCHI, Masayoshi, NAKAYAMA, Hajime
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Hepatology 1990
• Subjects: immunoreactive ornithine decarboxylase; ornithine decarboxylase
• ISSN: 0451-4203; 1881-3593; 1881-3593
• DOI: 10.2957/kanzo.31.610

We studied the effect of acute ethanol administration and sex difference in ornithine decarboxylase (ODC) activity, immunoreactive ODC (IR-ODC) protein, DNA synthesis and liver regeneration. Male and female adult Sprague-Dawley rats, weighting about 180g, had a 68% hepatectomy accompanied by oral administration of 5g/kg of 20% ethanol as ethanol group. Control groups were given same calories of glucose solution. Rats were killed and the liver remnant weight, total protein contents of liver, DNA synthesis, hepatic ODC activity and IR-ODC protein were analyzed. Serially at 0, 4, 8, 12, 24 hours after hepatectomy, IR-ODC protein was mesured by the methods of Hayasi (EIA assay). Liver weight, total protein contents and DNA synthesis were significantly decreased in female ethanol group. ODC activity and IR-ODC protein were significantly (p<0.05) decreased in the same female ethanol group at 4 hours after hepatectomy. These data suggest that the acute ethanol administration suppress the liver regeneration more promidently in female rats liver than in male livers. IR-ODC protein is a good marker of liver regeneration.