Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

• Published in: Chinese journal of oceanology and limnology Vol. 29; no. 4; pp. 912 - 916
• Main Author: 曹际娟 郑江 于兵 王秋艳 徐君怡 李爱峰
• Format: Journal Article
• Language: English
• Published: Heidelberg SP Science Press 01.07.2011; Springer Nature B.V
• Subjects: Algae; Bio-assays; Bioassays; Body weight; Earth and Environmental Science; Earth Sciences; Laboratories; Marine; Marine molluscs; Methods: Toxicity; Oceanography; Paralytic shellfish poisoning; Patinopecten yessoensis; Poisoning; Shellfish; Statistical methods; Technicians; Toxicity; Toxicity testing; Toxins; 实验室; 小鼠; 性能影响; 测定结果; 生物活性; 虾夷扇贝; 评价; 麻痹性贝毒素; China, People's Rep; INW, Bohai Sea; paralytic shellfish poisoning (PSP); inter-laboratory comparison; mouse bioassay
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-011-0521-9

An inter-laboratory comparison of the AOAC mouse bioassay for paralytic shellfish poisoning （PSP）toxicity in shellfish was carried out among 25 Chinese laboratories to examine the overall performance for PSP testing in China, and to analyze the main factors affecting the performance of this method. The toxic scallop Patinopecten yessoensis collected from coast of Bohai Sea, China, was used as a test sample in the comparison study. The results were reported and evaluated using robust statistical methods. The z scores showed that 80%, 8%, and 12% of laboratories reported satisfactory results, unsatisfactory results, and questionable results, respectively. This evaluation demonstrates that the PSP mouse bioassay is an appropriate method for screening and testing PSP toxicity in shellfish. However, it was found that the experience and skill of technicians, as well as the body weight and health status of mice being used significantly affected the accuracy of the method.