Evaluation of properties for Carbothane™ 3575A–based electrospun vascular grafts in vitro and in vivo

• Published in: Biomedical materials (Bristol) Vol. 19; no. 6; pp. 65012 - 65027
• Main Authors: Chernonosova, Vera S, Osipova, Olesia S, Nuankai, Zhou, Shundrina, Inna K, Murashov, Ivan S, Larichev, Yurii V, Karpenko, Andrey A, Laktionov, Pavel P
• Format: Journal Article
• Language: English
• Published: England IOP Publishing 01.11.2024
• Subjects: Carbothane™ 3575A; electrospinning; endothelial human cells; functioning; hemocompatibility; polyurethane-gelatin blend; small-diameter vascular graft; hemocompatibility; functioning in vivo; electrospinning; endothelial human cells; small-diameter vascular graft; polycarbonate-based polyurethane; Carbothane™ 3575A
• ISSN: 1748-6041; 1748-605X
• DOI: 10.1088/1748-605X/ad792d

Bioengineered vascular grafts (VGs) have emerged as a promising alternative to the treatment of damaged or occlusive vessels. It is thought that polyurethane (PU)-based scaffolds possess suitable hemocompatibility and biomechanics comparable to those of normal blood vessels. In this study, we investigated the properties of electrospun scaffolds comprising various blends of biostable polycarbonate-based PU (Carbothane™ 3575A) and gelatin. Scaffolds were characterized by scanning electron microscopy, infra-red spectroscopy, small-angle x-ray scattering, stress-loading tests, and interactions with primary human cells and blood. Data from in vitro experiments demonstrated that a scaffold produced from a blend of 5% Carbothane™ 3575A and 10% gelatin has proven to be a suitable material for fabricating a small-diameter VG. A comparative in vivo study of such VGs and expanded polytetrafluoroethylene (ePTFE) grafts implanted in the abdominal aorta of Wistar rats was performed. The data of intravital study and histological examination indicated that Carbothane-based electrospun grafts outclass ePTFE grafts and represent a promising device for preclinical studies to satisfy vascular surgery needs.