Development of DNA microarray for species identification of eels (Anguilliformes and Myxiniformes) in Korean fisheries markets

• Published in: Biochip journal Vol. 8; no. 4; pp. 310 - 316
• Main Authors: Park, Jung Youn, Cho, Hyunseok, Kang, Jung-Ha, Kim, Eun-Mi, An, Cheul Min, Kim, Ji-Hoon, Lee, Won Sun, Hwang, Seung Yong
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean BioChip Society (KBCS) 01.12.2014; Springer Nature B.V; 한국바이오칩학회
• Subjects: Biomedical Engineering and Bioengineering; Biotechnology; Chemistry; Chemistry and Materials Science; Consumers; Cytochrome-c oxidase; DNA chips; DNA microarrays; DNA probes; Eels; Fisheries; Original Article; Species; 생물공학; DNA microarray; oxidase subunit I; Species identification; Mitochondrial cytochrome
• ISSN: 1976-0280; 2092-7843
• DOI: 10.1007/s13206-014-8410-x

In Korea, eels are one of the most commercially valuable species and are prepared as a variety of dishes. Korean A. japonica is especially preferred by most consumers. However, the farming technology for this particular eel species has not been established yet, and the natural supply is insufficient because of environmental problems. As a result, the price of Korean A. japonica is expensive compared to other eel species because of the superior freshness and safety of the domestic product. Consequently, some importers and restaurant owners have profited significantly from selling imported eels to consumers as Korean A. japonica products. Hence, simpler, rapid and accurate techniques for eel species differentiation are needed. In this study, origin and species specific probes were designed by mitochondrial cytochrome c oxidase subunit I (COI) gene analysis and DNA microarray was developed for identifying different origins and species of eels. Using this technique, a total of 8 different eels were rapidly identified successfully.