Glycodelin gene expression in human peripheral white blood cells

• Published in: Irish journal of medical science Vol. 176; no. 2; pp. 101 - 104
• Main Authors: Lang, E. E., Venkatraman, G.
• Format: Journal Article
• Language: English
• Published: Ireland 01.06.2007
• Subjects: Female; Gene Expression - physiology; Glycodelin; Glycoproteins - genetics; Glycoproteins - metabolism; Humans; Leukocytes, Mononuclear - physiology; Male; Menstrual Cycle - physiology; Neovascularization, Physiologic - physiology; Pregnancy Proteins - genetics; Pregnancy Proteins - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured
• ISSN: 0021-1265; 1863-4362; 1863-4362
• DOI: 10.1007/s11845-007-0019-9

Glycodelin plays an important role in normal and abnormal reproductive growth. The in vitro properties of glycodelin have been studied in K562 cells. The role of glycodelin in vivo in the peripheral circulation has not been determined. To determine if circulating leukocytes in normal subjects express the glycodelin gene, with a view to understanding its contribution to various disease processes. RT-PCR analysis of mononuclear cells isolated from the blood of healthy volunteers was used to show glycodelin mRNA expression. Immunocytostaining was performed using anti-glycodelin antibody. PCR analysis illustrated the expression of the glycodelin gene in peripheral mononuclear cells. Glycodelin protein in these cells was confirmed with immunostaining. The presence of glycodelin in peripheral white blood cells was demonstrated. The ability of glycodelin to stimulate angiogenesis and act as an immunosuppressive agent could be an indicator of the role of peripheral leukocytes in various disease states.