Panacibacter ginsenosidivorans gen. nov., sp. nov., with ginsenoside converting activity isolated from soil of a ginseng field

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 10; pp. 4039 - 4045
• Main Authors: Siddiqi, Muhammad Zubair, Muhammad Shafi, Siddiqi, Choi, Kang Duk, Im, Wan-Taek
• Format: Journal Article
• Language: English
• Published: England 01.10.2016
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Ginsenosides - metabolism; Panax - microbiology; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Republic of Korea
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.001307

A ginsenoside-transforming bacterium, designated Gsoil 1550T, was isolated from soil of a ginseng field and subjected to a polyphasic taxonomic analysis. Colonies of strain Gsoil 1550T were yellow, of low convexity and with regular margin. Cells were long rods, 0.5-1.2 µm wide and 1.6-3 µm long. The isolate grew at 10-37 °C and at pH 5-9 on R2A agar medium; maximum growth occurred at 30 °C and pH 6-7. Phylogenetic study based on the 16S rRNA gene sequence positioned Gsoil 1550T in a distinct lineage in the family Chitinophagaceae, sharing 92.5-92.8 % sequence similarity with members of the closely related genera Terrimonas, Parafilimonas, Sediminibacterium and Parasegetibacter. Strain Gsoil 1550T contained menaquinone MK-7 as the predominant quinone, and iso-C15 : 0, iso-C17 : 0 3-OH, C16 : 0 and iso-C15 : 1 G as major fatty acids. The DNA G+C content was 44.6 mol%. Strain Gsoil 1550T could be distinguished from other members of the family Chitinophagaceae by a number of chemotaxonomic and phenotypic characteristics. The major polar lipid of strain Gsoil 1550T was phosphatidylethanolamine. Based on this polyphasic taxonomic analysis, strain Gsoil 1150T represents a novel species within a new genus, for which the name Panacibacter ginsenosidivorans gen. nov., sp. nov. is proposed. The type strain of Panacibacter ginsenosidivorans is Gsoil 1550T (=KCTC 12658T=JCM 31452T).