Spectroscopic analysis of AgI-DNA complex

It has recently been reoprted that C-C mismatches were able to recognize AgI specifically, and form AgI-mediated cytosine-cytosine base pairs (C-AgI-C pair). In this study, we explored DNA sequences, which are appropriate for structural studies of this new type of metal-mediated base pairs. For this...

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Bibliographic Details
Published inNucleic Acids Symposium Series Vol. 50; no. 1; pp. 199 - 200
Main Authors Oda, Shuji, Komuro, Tomoyuki, Kondo, Yoshinori, Ono, Akira, Tanaka, Yoshiyuki
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.11.2006
Subjects
Base Pair Mismatch
Cytosine - chemistry
DNA - chemistry
Silver - chemistry
Spectrophotometry, Ultraviolet
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ISSN0261-3166
1746-8272
1746-8272
DOI10.1093/nass/nrl099

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Summary:It has recently been reoprted that C-C mismatches were able to recognize AgI specifically, and form AgI-mediated cytosine-cytosine base pairs (C-AgI-C pair). In this study, we explored DNA sequences, which are appropriate for structural studies of this new type of metal-mediated base pairs. For this experiments, we synthesized four kinds of DNA duplexes including a single C-C mismatch. By using UV spectra, we performed titration experiments of DNA duplexes with AgNO3. Within the sequences we tested, it was found that the DNA duplex: 5′-CGCGCGTCC-3′ • 5′-GGACCCGCG-3′ showed 1:1 complexation of this DNA duplex and AgI. In addition, a DNA duplex: 5′-TAAATCTAATA-3′ • 5′-TATTACATTTA-3′ also exhibited a similar character. Therefore, these two DNA duplexes are interesting candidates for structural analyses.
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ISSN:0261-3166
1746-8272
1746-8272
DOI:10.1093/nass/nrl099