A B220+ CD117+ CD19± hematopoietic progenitor with potent lymphoid and myeloid developmental potential

• Published in: European journal of immunology Vol. 35; no. 7; pp. 2019 - 2030
• Main Authors: Balciunaite, Gina, Ceredig, Rod, Massa, Steffen, Rolink, Antonius G.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag 01.07.2005
• Subjects: Animals; Antigens - immunology; Antigens, CD19 - biosynthesis; Antigens, Ly; Antigens, Surface; B-Lymphocytes - cytology; B-Lymphocytes - immunology; B cell development; Cell Differentiation - immunology; Female; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - immunology; Lectins, C-Type; Leukocyte Common Antigens - biosynthesis; Lymphoid Tissue - cytology; Lymphoid Tissue - immunology; Lymphopoiesis; Mice; Mice, Congenic; Mice, Inbred BALB C; Mice, Inbred C57BL; Myeloid Cells - cytology; Myeloid Cells - immunology; Myeloid development; NK Cell Lectin-Like Receptor Subfamily B; Progenitor cells; Proteins - immunology; Proto-Oncogene Proteins c-kit - biosynthesis; T-Lymphocytes - cytology; T-Lymphocytes - immunology; T cell development
• ISSN: 0014-2980; 1521-4141
• DOI: 10.1002/eji.200526318

In this report, we identify in the bone marrow (BM) of normal mice a subpopulation of B220+ CD117+ CD19– NK1.1– cells with potent lymphoid and myeloid developmental potential. These cells represent 0.1–0.2% of nucleated BM cells. By limiting dilution analysis in the presence of the appropriate combination of stromal cells and cytokines, 1 in 5–10 sorted cells formed B cells, 1 in 10–15 formed T cells and 1 in 5–10 generated macrophages. When cultured on a mixture of OP9 stroma and OP9 stromal cells expressing the Notch ligand Delta‐like‐1, single cells generated both T and B cells. Following intravenous infusion, freshly sorted cells transiently reconstituted both the T and B cell progenitor compartments, generating cohorts of mature T and B lymphocytes. The relationship between B220+ CD117+ CD19– NK1.1– cells of wild‐type mice and other multi‐lineage BM progenitors is discussed. See accompanying commentary: http://dx.doi.org/10.1002/eji.200535106