The in-vitro and in-vivo radio labeling unfolds the vitellogenin protein expression site of Bombyx mori L as the ventral perivisceral fat body

• Published in: Journal of Asia-Pacific entomology Vol. 26; no. 2; pp. 102087 - 7
• Main Authors: Mohiadeen Batcha, M., Sajith Ahamed, A., Ur Rahman, M. Ashiq, Janarthanan, S., Krishnan, M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2023; 한국응용곤충학회
• Subjects: Bombyx mori L; Dorsal perivisceral fat body; S35 methionine; Ventral perivisceral fat body; Vitellogenin; 농학; PVF; Vg-H; Mmps; Ventral perivisceral fat body; DPV; Vitellogenin; DP; Bombyx mori L; Vg; PF; VP; S35 methionine; VPV; Vtn-L; Dorsal perivisceral fat body
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2023.102087

[Display omitted] •We subject dorsal perivisceral (DPV) and ventral perivisceral fat body (VPV), haemolymph for this radio labelling cell culture and incorporation experiment.•S35 methionine fluorography strongly proven the ventral perivisceral fat body (VPV) is the site of vitellogenin synthesis and not the dorsal peripheral fat body tissues (DPV).•200–205 kDa of vitellogenin protein was synthesized at two molecular weight regions, 180 kDa and 46 kDa. The present study aims to focus on the site of fat bodies involved in the differential expression of vitellogenin protein in Bombyx mori L which is not yet fully understood. Culturing fat bodies and analyzing in in vitro and in vivo approaches made it useful to study this pattern. In Bombyx mori, L. The vitellogenin protein is 200–205 kDa and resolves at two molecular weight regions that are at 180 kDa and 42 kDa. We subject dorsal perivisceral (DPV) and ventral perivisceral fat body (VPV), haemolymph for this radio labeling cell culture and incorporation experiment. Based on our in vitro and in vivo experiments of S35 methionine fluorography, Scanning densitometric, Western blot analysis and RT-PCR expression studies had strongly proven that the ventral perivisceral fat body is the significant and exclusive site of differential vitellogenin expression in silkworm, Bombyx mori but not in dorsal peripheral fat body tissues, haemolymph by radiolabeling studies. The present paper describes an experimental set-up based on the incubation of ventral perivisceral and dorsal perivisceral fat bodies and the measurement of vitellogenin with S35 protein labeling incorporation method. These findings from our lab were the first report proved by in vitro and in vivo labeling experiments.