Effect of loop on the stability of intramolecular triplex DNA

Conformation stabilities of intramolecular triple-helical DNAs (intra-tnplex DNAs: 5-d(TC)6-d(T)m-d(CT)6-d(C)n-d(AG)6-3’,where m and n are chosen to be 4 and 3) have been examined by molecular mechanics.The four intra-triplexes (H-DNAs) are compared with the mtermolecular triplexes d(TC)6 * d ( AG)6...

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Published inScience China. Chemistry Vol. 40; no. 6; pp. 650 - 656
Main Author 杨林静 白春礼 李任植 汪新文 王琛
Format Journal Article
LanguageEnglish
Published Heidelberg Springer Nature B.V 01.12.1997
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ISSN1674-7291
1006-9291
1869-1870
1862-2771
DOI10.1007/BF02875484

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Abstract Conformation stabilities of intramolecular triple-helical DNAs (intra-tnplex DNAs: 5-d(TC)6-d(T)m-d(CT)6-d(C)n-d(AG)6-3’,where m and n are chosen to be 4 and 3) have been examined by molecular mechanics.The four intra-triplexes (H-DNAs) are compared with the mtermolecular triplexes d(TC)6 * d ( AG)6 d(CT)6.The results revealed that loops do not significantly influence the mtratriplex conformations,loop conformations,however,depend partly on its length.Loop also makes strand Ⅱ of every intra-triplex DNA longer than that of the inter-triplex DNA.Most of residue sugar conformations of triple-helical DNAs are S-type,there aiso exist,however,N-type conformation and the conformations between S-type and N-type Possible models of the five triplex DNAs are presented.
AbstractList Conformation stabilities of intramolecular triple-helical DNAs (intra-triplex DNAs: 5′-d(TC)6- d(T)m-d(CT)6-d(C)n-d(AG)6-3′, wherem andn are chosen to be 4 and 3) have been examined by molecular mechanics. The four intra-triplexes (H-DNAs) are compared with the intermolecular triplexes d(TC)6 * d(AG)6 · d(CT)6. The results revealed that loops do not significantly influence the intratriplex conformations, loop conformations, however, depend partly on its length. Loop also makes strand II of every intra-triplex DNA longer than that of the inter-triplex DNA. Most of residue sugar conformations of triple-helical DNAs are S-type, there also exist, however, N-type conformation and the conformations between S-type and N-type. Possible models of the five triplex DNAs are presented.
Conformation stabilities of intramolecular triple-helical DNAs (intra-tnplex DNAs: 5-d(TC)6-d(T)m-d(CT)6-d(C)n-d(AG)6-3’,where m and n are chosen to be 4 and 3) have been examined by molecular mechanics.The four intra-triplexes (H-DNAs) are compared with the mtermolecular triplexes d(TC)6 * d ( AG)6 d(CT)6.The results revealed that loops do not significantly influence the mtratriplex conformations,loop conformations,however,depend partly on its length.Loop also makes strand Ⅱ of every intra-triplex DNA longer than that of the inter-triplex DNA.Most of residue sugar conformations of triple-helical DNAs are S-type,there aiso exist,however,N-type conformation and the conformations between S-type and N-type Possible models of the five triplex DNAs are presented.
Author 杨林静 白春礼 李任植 汪新文 王琛
AuthorAffiliation Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China China
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Notes YANG Linjing BAI Chunli LEE ImshikWANG Xinwen WANG Chen(Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080,China)
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Snippet Conformation stabilities of intramolecular triple-helical DNAs (intra-tnplex DNAs: 5-d(TC)6-d(T)m-d(CT)6-d(C)n-d(AG)6-3’,where m and n are chosen to be 4 and...
Conformation stabilities of intramolecular triple-helical DNAs (intra-triplex DNAs: 5′-d(TC)6- d(T)m-d(CT)6-d(C)n-d(AG)6-3′, wherem andn are chosen to be 4 and...
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SubjectTerms DNA
intra-triplex
loop
mechanics
molecular
stability
Title Effect of loop on the stability of intramolecular triplex DNA
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